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Dual expression of CCA-adding enzyme and RNase T in Escherichia coli generates a distinct cca growth phenotype with diverse applications

Correct synthesis and maintenance of functional tRNA 3′-CCA-ends is a crucial prerequisite for aminoacylation and must be achieved by the phylogenetically diverse group of tRNA nucleotidyltransferases. While numerous reports on the in vitro characterization exist, robust analysis under in vivo condi...

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Detalles Bibliográficos
Autores principales: Wellner, Karolin, Pöhler, Marie-Theres, Betat, Heike, Mörl, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468291/
https://www.ncbi.nlm.nih.gov/pubmed/30828718
http://dx.doi.org/10.1093/nar/gkz133