Engineering of a thermostable viral polymerase using metagenome-derived diversity for highly sensitive and specific RT-PCR

Reverse transcription is an essential initial step in the analysis of RNA for most PCR-based amplification and detection methods. Despite advancements in these technologies, efficient conversion of RNAs that form stable secondary structures and double-stranded RNA targets remains challenging as retr...

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Detalles Bibliográficos
Autores principales: Heller, Ryan C, Chung, Suhman, Crissy, Katarzyna, Dumas, Kyle, Schuster, David, Schoenfeld, Thomas W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468311/
https://www.ncbi.nlm.nih.gov/pubmed/30767012
http://dx.doi.org/10.1093/nar/gkz104

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468311/
https://www.ncbi.nlm.nih.gov/pubmed/30767012
http://dx.doi.org/10.1093/nar/gkz104

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