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Engineering of a thermostable viral polymerase using metagenome-derived diversity for highly sensitive and specific RT-PCR
Reverse transcription is an essential initial step in the analysis of RNA for most PCR-based amplification and detection methods. Despite advancements in these technologies, efficient conversion of RNAs that form stable secondary structures and double-stranded RNA targets remains challenging as retr...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468311/ https://www.ncbi.nlm.nih.gov/pubmed/30767012 http://dx.doi.org/10.1093/nar/gkz104 |