In-Vitro Characterization of mCerulean3_mRuby3 as a Novel FRET Pair with Favorable Bleed-Through Characteristics

In previous studies, we encountered substantial problems using the CFP_YFP Förster resonance energy transfer (FRET) pair to analyze protein proximity in the endoplasmic reticulum of live cells. Bleed-through of the donor emission into the FRET channel and overlap of the FRET emission wavelength with...

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Detalles Bibliográficos
Autores principales: Erismann-Ebner, Kira, Marowsky, Anne, Arand, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468510/
https://www.ncbi.nlm.nih.gov/pubmed/30823443
http://dx.doi.org/10.3390/bios9010033

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468510/
https://www.ncbi.nlm.nih.gov/pubmed/30823443
http://dx.doi.org/10.3390/bios9010033

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