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Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast
The CRISPR/Cas9 system enables the editing of genomes of numerous organisms through the induction of the double-strand breaks (DSB) at specific chromosomal targets. We improved the CRISPR/Cas9 system to ease the direct introduction of a point mutation or a tagging sequence into the chromosome by com...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Genetics Society of America
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469419/ https://www.ncbi.nlm.nih.gov/pubmed/30755408 http://dx.doi.org/10.1534/g3.118.200976 |