Fluorescent nucleic acid probe in droplets for bacterial sorting (FNAP-sort) as a high-throughput screening method for environmental bacteria with various growth rates

We have developed a new method for selectively sorting droplets containing growing bacteria using a fluorescence resonance energy transfer (FRET)-based RNA probe. Bacteria and the FRET-based RNA probe are encapsulated into nanoliter-scale droplets, which are incubated to allow for cell growth. The F...

Descripción completa

Detalles Bibliográficos
Autores principales: Ota, Yuri, Saito, Kanako, Takagi, Taeko, Matsukura, Satoko, Morita, Masamune, Tsuneda, Satoshi, Noda, Naohiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469844/
https://www.ncbi.nlm.nih.gov/pubmed/30995251
http://dx.doi.org/10.1371/journal.pone.0214533

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469844/
https://www.ncbi.nlm.nih.gov/pubmed/30995251
http://dx.doi.org/10.1371/journal.pone.0214533

Ejemplares similares