Production and Characterization of Recombinant Wild Type Uricase from Indonesian Coelacanth (L. menadoensis) and Improvement of Its Thermostability by In Silico Rational Design and Disulphide Bridges Engineering

The ideal therapeutic uricase (UOX) is expected to have the following properties; high expression level, high activity, high thermostability, high solubility and low immunogenicity. The latter property is believed to depend largely on sequence identity to the deduced human UOX (dH-UOX). Herein, we e...

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Detalles Bibliográficos
Autores principales: Yainoy, Sakda, Phuadraksa, Thanawat, Wichit, Sineewanlaya, Sompoppokakul, Maprang, Songtawee, Napat, Prachayasittikul, Virapong, Isarankura-Na-Ayudhya, Chartchalerm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471336/
https://www.ncbi.nlm.nih.gov/pubmed/30871218
http://dx.doi.org/10.3390/ijms20061269

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471336/
https://www.ncbi.nlm.nih.gov/pubmed/30871218
http://dx.doi.org/10.3390/ijms20061269

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