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Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis

Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method. The molecular weight distributions of the three oligosaccharides were 1.0–5.0 kDa, 0.4–1.4 kDa, and 1.0–7.0 kDa, respectively. The culture...

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Autores principales: Han, Zhen-Lian, Yang, Min, Fu, Xiao-Dan, Chen, Meng, Su, Qian, Zhao, Yuan-Hui, Mou, Hai-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471770/
https://www.ncbi.nlm.nih.gov/pubmed/30889794
http://dx.doi.org/10.3390/md17030173
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author Han, Zhen-Lian
Yang, Min
Fu, Xiao-Dan
Chen, Meng
Su, Qian
Zhao, Yuan-Hui
Mou, Hai-Jin
author_facet Han, Zhen-Lian
Yang, Min
Fu, Xiao-Dan
Chen, Meng
Su, Qian
Zhao, Yuan-Hui
Mou, Hai-Jin
author_sort Han, Zhen-Lian
collection PubMed
description Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method. The molecular weight distributions of the three oligosaccharides were 1.0–5.0 kDa, 0.4–1.4 kDa, and 1.0–7.0 kDa, respectively. The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition. Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium. AO could improve the gut microbiota composition by enriching the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium. Besides, KCO could increase the abundance of SCFA microbial producers and opportunistic pathogenic flora. Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation.
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spelling pubmed-64717702019-04-27 Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis Han, Zhen-Lian Yang, Min Fu, Xiao-Dan Chen, Meng Su, Qian Zhao, Yuan-Hui Mou, Hai-Jin Mar Drugs Article Alginate oligosaccharides (AlgO), agarose oligosaccharides (AO), and κ-carrageenan oligosaccharides (KCO) were obtained by specific enzymatic hydrolysis method. The molecular weight distributions of the three oligosaccharides were 1.0–5.0 kDa, 0.4–1.4 kDa, and 1.0–7.0 kDa, respectively. The culture medium was supplemented with the three oligosaccharides and fermented by pig fecal microbiota in vitro, for 24 h. Each oligosaccharide was capable of increasing the concentration of short-chain fatty acids (SCFAs), especially butyric acid, and altering the microbiota composition. Linear discriminant analysis effect size (LEfSe) analysis results showed that the opportunistic pathogenic bacteria Escherichia, Shigella, and Peptoniphilus, were significantly decreased in AlgO supplemented medium. AO could improve the gut microbiota composition by enriching the abundance of Ruminococcaceae, Coprococcus, Roseburia, and Faecalibacterium. Besides, KCO could increase the abundance of SCFA microbial producers and opportunistic pathogenic flora. Therefore, these results indicate that AlgO and AO can be used as gut microbial regulators and can potentially improve animal/human gastrointestinal health and prevent gut disease, whereas the physiological function of KCO needs further evaluation. MDPI 2019-03-18 /pmc/articles/PMC6471770/ /pubmed/30889794 http://dx.doi.org/10.3390/md17030173 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Zhen-Lian
Yang, Min
Fu, Xiao-Dan
Chen, Meng
Su, Qian
Zhao, Yuan-Hui
Mou, Hai-Jin
Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis
title Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis
title_full Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis
title_fullStr Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis
title_full_unstemmed Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis
title_short Evaluation of Prebiotic Potential of Three Marine Algae Oligosaccharides from Enzymatic Hydrolysis
title_sort evaluation of prebiotic potential of three marine algae oligosaccharides from enzymatic hydrolysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471770/
https://www.ncbi.nlm.nih.gov/pubmed/30889794
http://dx.doi.org/10.3390/md17030173
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