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Selection of reference genes for quantitative real-time PCR analysis in cucumber (Cucumis sativus L.), pumpkin (Cucurbita moschata Duch.) and cucumber–pumpkin grafted plants

BACKGROUND: Quantitative real-time PCR (qRT-PCR) is a commonly used high-throughput technique to measure mRNA transcript levels. The accuracy of this evaluation of gene expression depends on the use of optimal reference genes. Cucumber–pumpkin grafted plants, made by grafting a cucumber scion onto p...

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Detalles Bibliográficos
Autores principales: Miao, Li, Qin, Xing, Gao, Lihong, Li, Qing, Li, Shuzhen, He, Chaoxing, Li, Yansu, Yu, Xianchang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475253/
https://www.ncbi.nlm.nih.gov/pubmed/31024757
http://dx.doi.org/10.7717/peerj.6536