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Heterologous Expression of a Thermostable α-Glucosidase from Geobacillus sp. Strain HTA-462 by Escherichia coli and Its Potential Application for Isomaltose–Oligosaccharide Synthesis

Isomaltose–oligosaccharides (IMOs), as food ingredients with prebiotic functionality, can be prepared via enzymatic synthesis using α-glucosidase. In the present study, the α-glucosidase (GSJ) from Geobacillus sp. strain HTA-462 was cloned and expressed in Escherichia coli BL21 (DE3). Recombinant GS...

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Detalles Bibliográficos
Autores principales: Zhang, Fan, Wang, Weiyang, Bah, Fatoumata Binta Maci, Song, Chengcheng, Zhou, Yifa, Ji, Li, Yuan, Ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479687/
https://www.ncbi.nlm.nih.gov/pubmed/30974879
http://dx.doi.org/10.3390/molecules24071413