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Heterologous Expression of a Thermostable α-Glucosidase from Geobacillus sp. Strain HTA-462 by Escherichia coli and Its Potential Application for Isomaltose–Oligosaccharide Synthesis
Isomaltose–oligosaccharides (IMOs), as food ingredients with prebiotic functionality, can be prepared via enzymatic synthesis using α-glucosidase. In the present study, the α-glucosidase (GSJ) from Geobacillus sp. strain HTA-462 was cloned and expressed in Escherichia coli BL21 (DE3). Recombinant GS...
Autores principales: | Zhang, Fan, Wang, Weiyang, Bah, Fatoumata Binta Maci, Song, Chengcheng, Zhou, Yifa, Ji, Li, Yuan, Ye |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479687/ https://www.ncbi.nlm.nih.gov/pubmed/30974879 http://dx.doi.org/10.3390/molecules24071413 |
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