Preparation of Salvianolic Acid B Disodium Salt Considering the Water Extract Quality Standard

A preparation process of salvianolic acid B (SAB) disodium salt from Salvia miltiorrhiza Bunge (Danshen) is provided in this work. A water extract quality standard was also developed to estimate the influences of Danshen quality on SAB disodium salt quality at an early stage of the preparation proce...

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Autores principales: Ye, Tiantian, Qu, Haibin, Gong, Xingchu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479940/
https://www.ncbi.nlm.nih.gov/pubmed/30939842
http://dx.doi.org/10.3390/molecules24071269
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author Ye, Tiantian
Qu, Haibin
Gong, Xingchu
author_facet Ye, Tiantian
Qu, Haibin
Gong, Xingchu
author_sort Ye, Tiantian
collection PubMed
description A preparation process of salvianolic acid B (SAB) disodium salt from Salvia miltiorrhiza Bunge (Danshen) is provided in this work. A water extract quality standard was also developed to estimate the influences of Danshen quality on SAB disodium salt quality at an early stage of the preparation process. Crude SAB solution was obtained after water extraction, concentration, acidification, 1-butanol extraction, water washing, basification, and water back extraction. Extraction temperature, extraction pH, and back-extraction pH were identified to be key parameters for the preparation of crude SAB solution. These parameters were optimized with Box–Behnken designed experiments. Crude SAB solution was further purified with a chromatography process. AMBERCHROW CG161M resin was selected as the best adsorbent. SAB disodium salt could be obtained by drying the eluate. Considering the quality of Danshen may affect the purity and yield of SAB disodium salt, different batches of Danshen were used to prepare SAB disodium salt with the optimized parameters. Water extract indices of phenolic compound purity and phenolic compound yield were measured. By developing models between SAB disodium salt purity and yield with water extract indices, the quality standard of Danshen water extract was obtained. The application of water extract quality standards can improve the quality consistency of SAB disodium salt. The effects of different batches of Danshen raw materials on the final product could be evaluated at the beginning of production stages. The present method could prepare about five grams of high-purity SAB disodium salt (>95%) in one preparation cycle. The method reported in this work can also be used to develop process intermediate quality standards for other natural products.
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spelling pubmed-64799402019-04-30 Preparation of Salvianolic Acid B Disodium Salt Considering the Water Extract Quality Standard Ye, Tiantian Qu, Haibin Gong, Xingchu Molecules Article A preparation process of salvianolic acid B (SAB) disodium salt from Salvia miltiorrhiza Bunge (Danshen) is provided in this work. A water extract quality standard was also developed to estimate the influences of Danshen quality on SAB disodium salt quality at an early stage of the preparation process. Crude SAB solution was obtained after water extraction, concentration, acidification, 1-butanol extraction, water washing, basification, and water back extraction. Extraction temperature, extraction pH, and back-extraction pH were identified to be key parameters for the preparation of crude SAB solution. These parameters were optimized with Box–Behnken designed experiments. Crude SAB solution was further purified with a chromatography process. AMBERCHROW CG161M resin was selected as the best adsorbent. SAB disodium salt could be obtained by drying the eluate. Considering the quality of Danshen may affect the purity and yield of SAB disodium salt, different batches of Danshen were used to prepare SAB disodium salt with the optimized parameters. Water extract indices of phenolic compound purity and phenolic compound yield were measured. By developing models between SAB disodium salt purity and yield with water extract indices, the quality standard of Danshen water extract was obtained. The application of water extract quality standards can improve the quality consistency of SAB disodium salt. The effects of different batches of Danshen raw materials on the final product could be evaluated at the beginning of production stages. The present method could prepare about five grams of high-purity SAB disodium salt (>95%) in one preparation cycle. The method reported in this work can also be used to develop process intermediate quality standards for other natural products. MDPI 2019-04-01 /pmc/articles/PMC6479940/ /pubmed/30939842 http://dx.doi.org/10.3390/molecules24071269 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ye, Tiantian
Qu, Haibin
Gong, Xingchu
Preparation of Salvianolic Acid B Disodium Salt Considering the Water Extract Quality Standard
title Preparation of Salvianolic Acid B Disodium Salt Considering the Water Extract Quality Standard
title_full Preparation of Salvianolic Acid B Disodium Salt Considering the Water Extract Quality Standard
title_fullStr Preparation of Salvianolic Acid B Disodium Salt Considering the Water Extract Quality Standard
title_full_unstemmed Preparation of Salvianolic Acid B Disodium Salt Considering the Water Extract Quality Standard
title_short Preparation of Salvianolic Acid B Disodium Salt Considering the Water Extract Quality Standard
title_sort preparation of salvianolic acid b disodium salt considering the water extract quality standard
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479940/
https://www.ncbi.nlm.nih.gov/pubmed/30939842
http://dx.doi.org/10.3390/molecules24071269
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