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A simple non-toxic ethylene carbonate fluorescence in situ hybridization (EC-FISH) for simultaneous detection of repetitive DNA sequences and fluorescent bands in plants
The major drawbacks of standard plant fluorescence in situ hybridization (FISH) designed for double-stranded DNA probes include requirement for experimentally determined heat denaturation of chromosomes at high temperatures and at least overnight hybridization. Consequently, processing with chromoso...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Vienna
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482133/ https://www.ncbi.nlm.nih.gov/pubmed/30656455 http://dx.doi.org/10.1007/s00709-019-01345-7 |