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Performance of the Biomark HD real-time qPCR System (Fluidigm) for the detection of nasopharyngeal bacterial pathogens and Streptococcus pneumoniae typing

Traditional qPCR assays for pneumococcal detection and serotype characterization require large sample volume, is expensive and labor intensive. We aimed to develop a quantitative nanofluidic Fluidigm assay to overcome some of these shortcomings. A quantitative Fluidigm assay was established to detec...

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Detalles Bibliográficos
Autores principales: Olwagen, Courtney P., Adrian, Peter V., Madhi, Shabir A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482308/
https://www.ncbi.nlm.nih.gov/pubmed/31019272
http://dx.doi.org/10.1038/s41598-019-42846-y