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Measuring intracellular concentration of hydrogen peroxide with the use of genetically encoded H(2)O(2) biosensor HyPer
In this study, we propose a method for quantification of average hydrogen peroxide concentration within a living cell that is based on the use of genetically encoded H(2)O(2) biosensor HyPer. The method utilizes flow cytometric measurements of HyPer fluorescence in H(2)O(2)-exposed cells to analyze...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482347/ https://www.ncbi.nlm.nih.gov/pubmed/31030065 http://dx.doi.org/10.1016/j.redox.2019.101200 |