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Genetic diversity of cytochrome P450 3A with different metabolic activity in domestic cats
Knowledge on genetic polymorphisms of metabolising enzymes including cytochrome P450 (CYP) is very limited in cats. We investigated polymorphisms in CYP3A131, one of the major CYP isoforms in the feline liver and small intestine. Eight non-synonymous variants and one synonymous variant of feline CYP...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483910/ https://www.ncbi.nlm.nih.gov/pubmed/30828039 http://dx.doi.org/10.1292/jvms.18-0692 |
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author | SUGIYAMA, Souta UNO, Yasuhiro AMANO, Tomoko KITAZAWA, Takio TERAOKA, Hiroki |
author_facet | SUGIYAMA, Souta UNO, Yasuhiro AMANO, Tomoko KITAZAWA, Takio TERAOKA, Hiroki |
author_sort | SUGIYAMA, Souta |
collection | PubMed |
description | Knowledge on genetic polymorphisms of metabolising enzymes including cytochrome P450 (CYP) is very limited in cats. We investigated polymorphisms in CYP3A131, one of the major CYP isoforms in the feline liver and small intestine. Eight non-synonymous variants and one synonymous variant of feline CYP3A131 were identified in 29 cats. A major non-synonymous type was not observed. Metabolic parameters (Km and Vmax) of dibenzylfluorescein hydroxylation were ranged within about 2 times for the identified non-synonymous variants by using a heterologous coexpression system of CYP3A131 and feline cytochrome P450 reductase in Escherichia coli. The results confirmed the polymorphic nature of CYP3A131 as a basis for effective application of medicines and prevention of adverse reactions in the treatment of domestic cats. |
format | Online Article Text |
id | pubmed-6483910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64839102019-04-30 Genetic diversity of cytochrome P450 3A with different metabolic activity in domestic cats SUGIYAMA, Souta UNO, Yasuhiro AMANO, Tomoko KITAZAWA, Takio TERAOKA, Hiroki J Vet Med Sci Toxicology Knowledge on genetic polymorphisms of metabolising enzymes including cytochrome P450 (CYP) is very limited in cats. We investigated polymorphisms in CYP3A131, one of the major CYP isoforms in the feline liver and small intestine. Eight non-synonymous variants and one synonymous variant of feline CYP3A131 were identified in 29 cats. A major non-synonymous type was not observed. Metabolic parameters (Km and Vmax) of dibenzylfluorescein hydroxylation were ranged within about 2 times for the identified non-synonymous variants by using a heterologous coexpression system of CYP3A131 and feline cytochrome P450 reductase in Escherichia coli. The results confirmed the polymorphic nature of CYP3A131 as a basis for effective application of medicines and prevention of adverse reactions in the treatment of domestic cats. The Japanese Society of Veterinary Science 2019-03-04 2019-04 /pmc/articles/PMC6483910/ /pubmed/30828039 http://dx.doi.org/10.1292/jvms.18-0692 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Toxicology SUGIYAMA, Souta UNO, Yasuhiro AMANO, Tomoko KITAZAWA, Takio TERAOKA, Hiroki Genetic diversity of cytochrome P450 3A with different metabolic activity in domestic cats |
title | Genetic diversity of cytochrome P450 3A with different metabolic activity in
domestic cats |
title_full | Genetic diversity of cytochrome P450 3A with different metabolic activity in
domestic cats |
title_fullStr | Genetic diversity of cytochrome P450 3A with different metabolic activity in
domestic cats |
title_full_unstemmed | Genetic diversity of cytochrome P450 3A with different metabolic activity in
domestic cats |
title_short | Genetic diversity of cytochrome P450 3A with different metabolic activity in
domestic cats |
title_sort | genetic diversity of cytochrome p450 3a with different metabolic activity in
domestic cats |
topic | Toxicology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483910/ https://www.ncbi.nlm.nih.gov/pubmed/30828039 http://dx.doi.org/10.1292/jvms.18-0692 |
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