High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases

The development of site-specific recombinases (SSRs) as genome editing agents is limited by the difficulty of altering their native DNA specificities. Here we describe Rec-seq, a method for revealing the DNA specificity determinants and potential off-target substrates of SSRs in a comprehensive and...

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Detalles Bibliográficos
Autores principales: Bessen, Jeffrey L., Afeyan, Lena K., Dančík, Vlado, Koblan, Luke W., Thompson, David B., Leichner, Chas, Clemons, Paul A., Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6486577/
https://www.ncbi.nlm.nih.gov/pubmed/31028261
http://dx.doi.org/10.1038/s41467-019-09987-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6486577/
https://www.ncbi.nlm.nih.gov/pubmed/31028261
http://dx.doi.org/10.1038/s41467-019-09987-0

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