Enhanced Cas12a editing in mammalian cells and zebrafish

Type V CRISPR–Cas12a systems provide an alternate nuclease platform to Cas9, with potential advantages for specific genome editing applications. Here we describe improvements to the Cas12a system that facilitate efficient targeted mutagenesis in mammalian cells and zebrafish embryos. We show that en...

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Detalles Bibliográficos
Autores principales: Liu, Pengpeng, Luk, Kevin, Shin, Masahiro, Idrizi, Feston, Kwok, Samantha, Roscoe, Benjamin, Mintzer, Esther, Suresh, Sneha, Morrison, Kyle, Frazão, Josias B, Bolukbasi, Mehmet Fatih, Ponnienselvan, Karthikeyan, Luban, Jeremy, Zhu, Lihua Julie, Lawson, Nathan D, Wolfe, Scot A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6486634/
https://www.ncbi.nlm.nih.gov/pubmed/30892626
http://dx.doi.org/10.1093/nar/gkz184

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6486634/
https://www.ncbi.nlm.nih.gov/pubmed/30892626
http://dx.doi.org/10.1093/nar/gkz184

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