Cargando…

Enhanced Cas12a editing in mammalian cells and zebrafish

Type V CRISPR–Cas12a systems provide an alternate nuclease platform to Cas9, with potential advantages for specific genome editing applications. Here we describe improvements to the Cas12a system that facilitate efficient targeted mutagenesis in mammalian cells and zebrafish embryos. We show that en...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Pengpeng, Luk, Kevin, Shin, Masahiro, Idrizi, Feston, Kwok, Samantha, Roscoe, Benjamin, Mintzer, Esther, Suresh, Sneha, Morrison, Kyle, Frazão, Josias B, Bolukbasi, Mehmet Fatih, Ponnienselvan, Karthikeyan, Luban, Jeremy, Zhu, Lihua Julie, Lawson, Nathan D, Wolfe, Scot A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2019
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6486634/ https://www.ncbi.nlm.nih.gov/pubmed/30892626 http://dx.doi.org/10.1093/nar/gkz184

Ejemplares similares

Regulation of RNA editing by intracellular acidification
por: Malik, Turnee N, et al.
Publicado: (2021)

Coordination between aminoacylation and editing to protect against proteotoxicity
por: Zhang, Hong, et al.
Publicado: (2023)

Impact of alanyl-tRNA synthetase editing deficiency in yeast
por: Zhang, Hong, et al.
Publicado: (2021)

Using defined finger–finger interfaces as units of assembly for constructing zinc-finger nucleases
por: Zhu, Cong, et al.
Publicado: (2013)

The ADAR RNA editing enzyme controls neuronal excitability in Drosophila melanogaster
por: Li, Xianghua, et al.
Publicado: (2014)

Editing activity for eliminating mischarged tRNAs is essential in mammalian mitochondria
por: Hilander, Taru, et al.
Publicado: (2018)

DNA editing in DNA/RNA hybrids by adenosine deaminases that act on RNA
por: Zheng, Yuxuan, et al.
Publicado: (2017)

In-depth assessment of the PAM compatibility and editing activities of Cas9 variants
por: Zhang, Weiwei, et al.
Publicado: (2021)

Recognition of duplex RNA by the deaminase domain of the RNA editing enzyme ADAR2
por: Phelps, Kelly J., et al.
Publicado: (2015)

Negative catalysis by the editing domain of class I aminoacyl-tRNA synthetases
por: Zivkovic, Igor, et al.
Publicado: (2022)

Nucleoside analog studies indicate mechanistic differences between RNA-editing adenosine deaminases
por: Mizrahi, Rena A., et al.
Publicado: (2012)

Oxidation alters the architecture of the phenylalanyl-tRNA synthetase editing domain to confer hyperaccuracy
por: Srinivas, Pooja, et al.
Publicado: (2021)

RNA Editing TUTase 1: structural foundation of substrate recognition, complex interactions and drug targeting
por: Rajappa-Titu, Lional, et al.
Publicado: (2016)

Editing of misaminoacylated tRNA controls the sensitivity of amino acid stress responses in Saccharomyces cerevisiae
por: Mohler, Kyle, et al.
Publicado: (2017)

Nitrosative stress inhibits aminoacylation and editing activities of mitochondrial threonyl-tRNA synthetase by S-nitrosation
por: Zheng, Wen-Qiang, et al.
Publicado: (2020)

Discovery of potent and versatile CRISPR–Cas9 inhibitors engineered for chemically controllable genome editing
por: Song, Guoxu, et al.
Publicado: (2022)

Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase
por: Chen, Xin, et al.
Publicado: (2011)

A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity
por: Mussolino, Claudio, et al.
Publicado: (2011)

Crucial role of the C-terminal domain of Mycobacterium tuberculosis leucyl-tRNA synthetase in aminoacylation and editing
por: Hu, Qing-Hua, et al.
Publicado: (2013)

Progressive engineering of a homing endonuclease genome editing reagent for the murine X-linked immunodeficiency locus
por: Wang, Yupeng, et al.
Publicado: (2014)

A threonyl-tRNA synthetase-like protein has tRNA aminoacylation and editing activities
por: Chen, Yun, et al.
Publicado: (2018)

Stereospecificity control in aminoacyl-tRNA-synthetases: new evidence of d-amino acids activation and editing
por: Rybak, Mariia Yu, et al.
Publicado: (2019)

Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells
por: Tao, Rui, et al.
Publicado: (2022)

A protein–protein interaction underlies the molecular basis for substrate recognition by an adenosine-to-inosine RNA-editing enzyme
por: Rajendren, Suba, et al.
Publicado: (2018)

A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells
por: Tu, Mengjun, et al.
Publicado: (2017)

Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain
por: Ji, Quan-Quan, et al.
Publicado: (2017)

Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency
por: Ponnienselvan, Karthikeyan, et al.
Publicado: (2023)

Elucidating the mechanism of DNA-dependent ATP hydrolysis mediated by DNA-dependent ATPase A, a member of the SWI2/SNF2 protein family
por: Nongkhlaw, Macmillan, et al.
Publicado: (2009)

Preferred WMSA catalytic mechanism of the nucleotidyl transfer reaction in human DNA polymerase κ elucidates error-free bypass of a bulky DNA lesion
por: Lior-Hoffmann, Lee, et al.
Publicado: (2012)

Homing endonuclease I-CreI derivatives with novel DNA target specificities
por: Rosen, Laura E., et al.
Publicado: (2006)

High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination
por: Yuen, Chaya T L, et al.
Publicado: (2022)

Magnesium-binding studies reveal fundamental differences between closely related RNA triphosphatases
por: Soulière, Marie F., et al.
Publicado: (2008)

Mechanisms of covalent self-assembly of the Azoarcus ribozyme from four fragment oligonucleotides
por: Draper, Will E., et al.
Publicado: (2008)

Cordycepin-hypersensitive growth links elevated polyphosphate levels to inhibition of poly(A) polymerase in Saccharomyces cerevisiae
por: Holbein, Sandra, et al.
Publicado: (2008)

The Leu22Pro tumor-associated variant of DNA polymerase beta is dRP lyase deficient
por: Dalal, Shibani, et al.
Publicado: (2008)

DNA polymerase kappa produces interrupted mutations and displays polar pausing within mononucleotide microsatellite sequences
por: Hile, Suzanne E., et al.
Publicado: (2008)

The N-terminal domain of TWINKLE contributes to single-stranded DNA binding and DNA helicase activities
por: Farge, Géraldine, et al.
Publicado: (2008)

The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases
por: Leulliot, Nicolas, et al.
Publicado: (2008)

Structure–function analysis of the RNA polymerase cleft loops elucidates initial transcription, DNA unwinding and RNA displacement
por: Naji, Souad, et al.
Publicado: (2008)

Site specific phosphorylation of yeast RNA polymerase I
por: Gerber, Jochen, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_vjdnssbg679vcberja7k2a6tvr