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Two Approaches to Enhance the Processivity and Salt Tolerance of Staphylococcus aureus DNA Polymerase
In this article, two engineering-strategies were carried out to enhance the processivity of the DNA polymerase used in recombinase polymerase amplification (RPA). We demonstrate that covalent linkage of a non-specific, double-stranded DNA binding protein, Sso7d, to the large fragment of Staphylococc...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6486907/ https://www.ncbi.nlm.nih.gov/pubmed/30759302 http://dx.doi.org/10.1007/s10930-019-09818-7 |