Cargando…

Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli

AIM: This study aimed to estimate the enumeration of total bacteria and coliform on teat skin from dairy cows and evaluate the efficacy of the natural rice gel containing 5% v/v lactic acid (NGL) against Escherichia coli standard and field strains isolated from bovine teat skin. MATERIALS AND METHOD...

Descripción completa

Detalles Bibliográficos
Autores principales: Chotigarpa, Rinrada, Lampang, Kannika Na, Pikulkaew, Surachai, Okonogi, Siriporn, Silman, Pirote, Mektrirat, Raktham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487238/
https://www.ncbi.nlm.nih.gov/pubmed/31089309
http://dx.doi.org/10.14202/vetworld.2019.397-401
_version_ 1783414462047322112
author Chotigarpa, Rinrada
Lampang, Kannika Na
Pikulkaew, Surachai
Okonogi, Siriporn
Silman, Pirote
Mektrirat, Raktham
author_facet Chotigarpa, Rinrada
Lampang, Kannika Na
Pikulkaew, Surachai
Okonogi, Siriporn
Silman, Pirote
Mektrirat, Raktham
author_sort Chotigarpa, Rinrada
collection PubMed
description AIM: This study aimed to estimate the enumeration of total bacteria and coliform on teat skin from dairy cows and evaluate the efficacy of the natural rice gel containing 5% v/v lactic acid (NGL) against Escherichia coli standard and field strains isolated from bovine teat skin. MATERIALS AND METHODS: A total of 100 bacterial teat skin samples (25 cows) were collected from dairy cows in smallholder farm. The cows were housed in freestall barns. The colonization of total bacteria and E. coli on teat skin was measured by 3M Petrifilm method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of lactic acid were evaluated for reference strain of E. coli ATCC 25922 and two field strains of E. coli. The natural teat sanitizer was formulated using 5% NGL with modified rice gel. In vitro antiseptic efficacy of 5% NGL was determined by time-kill kinetic assay. E. coli morphology after exposure with 5% NGL was examined under a scanning electron microscope (SEM). RESULTS: The total bacteria and coliform counts from bovine teat skin were 2.11×10(4) and 1.54×10(1) colony-forming units/ml, respectively. The MIC and MBC of lactic acid on the tested bacteria were 0.5% v/v. The natural teat dip was successfully prepared with minimum change in consistency after 1 year of storage at 4°C. The reduction rate of 5% NGL on E. coli ATCC 25922 and field strain showed 32.77% and 27.58%, respectively. An appearance under SEM of non-viable E. coli after being incubated with 5% NGL clearly showed atypical form and rough surface cell membrane. CONCLUSION: The rice gel containing 5% v/v lactic acid is a promising preparation as a natural teat antiseptic for reducing bacteria on teat skin. It was shown to be effective against E. coli causing bovine mastitis in dairy cows.
format Online
Article
Text
id pubmed-6487238
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-64872382019-05-14 Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli Chotigarpa, Rinrada Lampang, Kannika Na Pikulkaew, Surachai Okonogi, Siriporn Silman, Pirote Mektrirat, Raktham Vet World Research Article AIM: This study aimed to estimate the enumeration of total bacteria and coliform on teat skin from dairy cows and evaluate the efficacy of the natural rice gel containing 5% v/v lactic acid (NGL) against Escherichia coli standard and field strains isolated from bovine teat skin. MATERIALS AND METHODS: A total of 100 bacterial teat skin samples (25 cows) were collected from dairy cows in smallholder farm. The cows were housed in freestall barns. The colonization of total bacteria and E. coli on teat skin was measured by 3M Petrifilm method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of lactic acid were evaluated for reference strain of E. coli ATCC 25922 and two field strains of E. coli. The natural teat sanitizer was formulated using 5% NGL with modified rice gel. In vitro antiseptic efficacy of 5% NGL was determined by time-kill kinetic assay. E. coli morphology after exposure with 5% NGL was examined under a scanning electron microscope (SEM). RESULTS: The total bacteria and coliform counts from bovine teat skin were 2.11×10(4) and 1.54×10(1) colony-forming units/ml, respectively. The MIC and MBC of lactic acid on the tested bacteria were 0.5% v/v. The natural teat dip was successfully prepared with minimum change in consistency after 1 year of storage at 4°C. The reduction rate of 5% NGL on E. coli ATCC 25922 and field strain showed 32.77% and 27.58%, respectively. An appearance under SEM of non-viable E. coli after being incubated with 5% NGL clearly showed atypical form and rough surface cell membrane. CONCLUSION: The rice gel containing 5% v/v lactic acid is a promising preparation as a natural teat antiseptic for reducing bacteria on teat skin. It was shown to be effective against E. coli causing bovine mastitis in dairy cows. Veterinary World 2019 2019-03-15 /pmc/articles/PMC6487238/ /pubmed/31089309 http://dx.doi.org/10.14202/vetworld.2019.397-401 Text en Copyright: © Chotigarpa, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Chotigarpa, Rinrada
Lampang, Kannika Na
Pikulkaew, Surachai
Okonogi, Siriporn
Silman, Pirote
Mektrirat, Raktham
Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli
title Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli
title_full Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli
title_fullStr Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli
title_full_unstemmed Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli
title_short Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli
title_sort antiseptic effect of natural teat dip containing lactic acid against mastitis-causing escherichia coli
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487238/
https://www.ncbi.nlm.nih.gov/pubmed/31089309
http://dx.doi.org/10.14202/vetworld.2019.397-401
work_keys_str_mv AT chotigarparinrada antisepticeffectofnaturalteatdipcontaininglacticacidagainstmastitiscausingescherichiacoli
AT lampangkannikana antisepticeffectofnaturalteatdipcontaininglacticacidagainstmastitiscausingescherichiacoli
AT pikulkaewsurachai antisepticeffectofnaturalteatdipcontaininglacticacidagainstmastitiscausingescherichiacoli
AT okonogisiriporn antisepticeffectofnaturalteatdipcontaininglacticacidagainstmastitiscausingescherichiacoli
AT silmanpirote antisepticeffectofnaturalteatdipcontaininglacticacidagainstmastitiscausingescherichiacoli
AT mektriratraktham antisepticeffectofnaturalteatdipcontaininglacticacidagainstmastitiscausingescherichiacoli