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Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli
AIM: This study aimed to estimate the enumeration of total bacteria and coliform on teat skin from dairy cows and evaluate the efficacy of the natural rice gel containing 5% v/v lactic acid (NGL) against Escherichia coli standard and field strains isolated from bovine teat skin. MATERIALS AND METHOD...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487238/ https://www.ncbi.nlm.nih.gov/pubmed/31089309 http://dx.doi.org/10.14202/vetworld.2019.397-401 |
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author | Chotigarpa, Rinrada Lampang, Kannika Na Pikulkaew, Surachai Okonogi, Siriporn Silman, Pirote Mektrirat, Raktham |
author_facet | Chotigarpa, Rinrada Lampang, Kannika Na Pikulkaew, Surachai Okonogi, Siriporn Silman, Pirote Mektrirat, Raktham |
author_sort | Chotigarpa, Rinrada |
collection | PubMed |
description | AIM: This study aimed to estimate the enumeration of total bacteria and coliform on teat skin from dairy cows and evaluate the efficacy of the natural rice gel containing 5% v/v lactic acid (NGL) against Escherichia coli standard and field strains isolated from bovine teat skin. MATERIALS AND METHODS: A total of 100 bacterial teat skin samples (25 cows) were collected from dairy cows in smallholder farm. The cows were housed in freestall barns. The colonization of total bacteria and E. coli on teat skin was measured by 3M Petrifilm method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of lactic acid were evaluated for reference strain of E. coli ATCC 25922 and two field strains of E. coli. The natural teat sanitizer was formulated using 5% NGL with modified rice gel. In vitro antiseptic efficacy of 5% NGL was determined by time-kill kinetic assay. E. coli morphology after exposure with 5% NGL was examined under a scanning electron microscope (SEM). RESULTS: The total bacteria and coliform counts from bovine teat skin were 2.11×10(4) and 1.54×10(1) colony-forming units/ml, respectively. The MIC and MBC of lactic acid on the tested bacteria were 0.5% v/v. The natural teat dip was successfully prepared with minimum change in consistency after 1 year of storage at 4°C. The reduction rate of 5% NGL on E. coli ATCC 25922 and field strain showed 32.77% and 27.58%, respectively. An appearance under SEM of non-viable E. coli after being incubated with 5% NGL clearly showed atypical form and rough surface cell membrane. CONCLUSION: The rice gel containing 5% v/v lactic acid is a promising preparation as a natural teat antiseptic for reducing bacteria on teat skin. It was shown to be effective against E. coli causing bovine mastitis in dairy cows. |
format | Online Article Text |
id | pubmed-6487238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-64872382019-05-14 Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli Chotigarpa, Rinrada Lampang, Kannika Na Pikulkaew, Surachai Okonogi, Siriporn Silman, Pirote Mektrirat, Raktham Vet World Research Article AIM: This study aimed to estimate the enumeration of total bacteria and coliform on teat skin from dairy cows and evaluate the efficacy of the natural rice gel containing 5% v/v lactic acid (NGL) against Escherichia coli standard and field strains isolated from bovine teat skin. MATERIALS AND METHODS: A total of 100 bacterial teat skin samples (25 cows) were collected from dairy cows in smallholder farm. The cows were housed in freestall barns. The colonization of total bacteria and E. coli on teat skin was measured by 3M Petrifilm method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of lactic acid were evaluated for reference strain of E. coli ATCC 25922 and two field strains of E. coli. The natural teat sanitizer was formulated using 5% NGL with modified rice gel. In vitro antiseptic efficacy of 5% NGL was determined by time-kill kinetic assay. E. coli morphology after exposure with 5% NGL was examined under a scanning electron microscope (SEM). RESULTS: The total bacteria and coliform counts from bovine teat skin were 2.11×10(4) and 1.54×10(1) colony-forming units/ml, respectively. The MIC and MBC of lactic acid on the tested bacteria were 0.5% v/v. The natural teat dip was successfully prepared with minimum change in consistency after 1 year of storage at 4°C. The reduction rate of 5% NGL on E. coli ATCC 25922 and field strain showed 32.77% and 27.58%, respectively. An appearance under SEM of non-viable E. coli after being incubated with 5% NGL clearly showed atypical form and rough surface cell membrane. CONCLUSION: The rice gel containing 5% v/v lactic acid is a promising preparation as a natural teat antiseptic for reducing bacteria on teat skin. It was shown to be effective against E. coli causing bovine mastitis in dairy cows. Veterinary World 2019 2019-03-15 /pmc/articles/PMC6487238/ /pubmed/31089309 http://dx.doi.org/10.14202/vetworld.2019.397-401 Text en Copyright: © Chotigarpa, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Chotigarpa, Rinrada Lampang, Kannika Na Pikulkaew, Surachai Okonogi, Siriporn Silman, Pirote Mektrirat, Raktham Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli |
title | Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli |
title_full | Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli |
title_fullStr | Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli |
title_full_unstemmed | Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli |
title_short | Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli |
title_sort | antiseptic effect of natural teat dip containing lactic acid against mastitis-causing escherichia coli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487238/ https://www.ncbi.nlm.nih.gov/pubmed/31089309 http://dx.doi.org/10.14202/vetworld.2019.397-401 |
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