High-yield Production of Granulocyte-macrophage Colony-stimulating Factor in E. coli BL21 (DE3) By an Auto-induction Strategy

A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression...

Descripción completa

Detalles Bibliográficos
Autores principales: Malekian, Raziyeh, Jahanian-Najafabadi, Ali, Moazen, Fatemeh, Ghavimi, Reza, Mohammadi, Elmira, Akbari, Vajihe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487437/
https://www.ncbi.nlm.nih.gov/pubmed/31089381

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487437/
https://www.ncbi.nlm.nih.gov/pubmed/31089381

Ejemplares similares