Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer

Long noncoding RNAs (lncRNAs) are frequently precursor RNAs of microRNAs (miRNAs) or act as competing endogenous RNAs (ceRNAs) to interact with miRNAs. To better understand the shared impact of lncRNAs and miRNAs in the regulatory post‐transcriptional network, we focused here on the relationships be...

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Autores principales: Müller, Volkmar, Oliveira‐Ferrer, Leticia, Steinbach, Bettina, Pantel, Klaus, Schwarzenbach, Heidi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487715/
https://www.ncbi.nlm.nih.gov/pubmed/30803129
http://dx.doi.org/10.1002/1878-0261.12472
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author Müller, Volkmar
Oliveira‐Ferrer, Leticia
Steinbach, Bettina
Pantel, Klaus
Schwarzenbach, Heidi
author_facet Müller, Volkmar
Oliveira‐Ferrer, Leticia
Steinbach, Bettina
Pantel, Klaus
Schwarzenbach, Heidi
author_sort Müller, Volkmar
collection PubMed
description Long noncoding RNAs (lncRNAs) are frequently precursor RNAs of microRNAs (miRNAs) or act as competing endogenous RNAs (ceRNAs) to interact with miRNAs. To better understand the shared impact of lncRNAs and miRNAs in the regulatory post‐transcriptional network, we focused here on the relationships between (a) lncRNA H19 and miR‐675, (b) NEAT1 and miR‐204, and (c) HOTAIR and miR‐331 in plasma of early breast cancer (BC) patients. We quantified each RNA in plasma samples of 63 BC patients and 10 healthy women by quantitative real‐time PCR. In cell culture experiments, the influence of these noncoding RNAs (ncRNAs) on proliferation and apoptosis of BC cell line MCF‐7 was examined. Plasma levels of H19 (P = 0.030), NEAT1 (P = 0.030), and miR‐331 (P = 0.012) were deregulated in BC patients compared with healthy women. In both cohorts, the concentrations of H19 correlated with those of miR‐675 (P = 0.0001). Higher H19 (P = 0.001) along with lower miR‐675 (P = 0.007) levels and higher miR‐204 (P = 0.017) along with lower NEAT1 (P = 0.030) levels were detected in plasma of HER2‐positive patients compared with the other BC subgroups. Whereas the expression of HOTAIR was below the detection level, miR‐331 levels correlated with nodal status (P = 0.002) and recurrence (P = 0.012). In cell culture experiments, a competitive impact on cell proliferation and apoptosis by these ncRNAs was also documented. Our findings describe a relationship of the plasma levels of H19/miR‐675 and NEAT1/miR‐204 in the different BC subtypes; in addition, they reveal an interplay between these lncRNAs and miRNAs in the regulatory network in MCF‐7 cells, which should also be considered in the search for new diagnostic and therapeutic markers.
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spelling pubmed-64877152019-05-07 Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer Müller, Volkmar Oliveira‐Ferrer, Leticia Steinbach, Bettina Pantel, Klaus Schwarzenbach, Heidi Mol Oncol Research Articles Long noncoding RNAs (lncRNAs) are frequently precursor RNAs of microRNAs (miRNAs) or act as competing endogenous RNAs (ceRNAs) to interact with miRNAs. To better understand the shared impact of lncRNAs and miRNAs in the regulatory post‐transcriptional network, we focused here on the relationships between (a) lncRNA H19 and miR‐675, (b) NEAT1 and miR‐204, and (c) HOTAIR and miR‐331 in plasma of early breast cancer (BC) patients. We quantified each RNA in plasma samples of 63 BC patients and 10 healthy women by quantitative real‐time PCR. In cell culture experiments, the influence of these noncoding RNAs (ncRNAs) on proliferation and apoptosis of BC cell line MCF‐7 was examined. Plasma levels of H19 (P = 0.030), NEAT1 (P = 0.030), and miR‐331 (P = 0.012) were deregulated in BC patients compared with healthy women. In both cohorts, the concentrations of H19 correlated with those of miR‐675 (P = 0.0001). Higher H19 (P = 0.001) along with lower miR‐675 (P = 0.007) levels and higher miR‐204 (P = 0.017) along with lower NEAT1 (P = 0.030) levels were detected in plasma of HER2‐positive patients compared with the other BC subgroups. Whereas the expression of HOTAIR was below the detection level, miR‐331 levels correlated with nodal status (P = 0.002) and recurrence (P = 0.012). In cell culture experiments, a competitive impact on cell proliferation and apoptosis by these ncRNAs was also documented. Our findings describe a relationship of the plasma levels of H19/miR‐675 and NEAT1/miR‐204 in the different BC subtypes; in addition, they reveal an interplay between these lncRNAs and miRNAs in the regulatory network in MCF‐7 cells, which should also be considered in the search for new diagnostic and therapeutic markers. John Wiley and Sons Inc. 2019-03-14 2019-05 /pmc/articles/PMC6487715/ /pubmed/30803129 http://dx.doi.org/10.1002/1878-0261.12472 Text en © 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Müller, Volkmar
Oliveira‐Ferrer, Leticia
Steinbach, Bettina
Pantel, Klaus
Schwarzenbach, Heidi
Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer
title Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer
title_full Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer
title_fullStr Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer
title_full_unstemmed Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer
title_short Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer
title_sort interplay of lncrna h19/mir‐675 and lncrna neat1/mir‐204 in breast cancer
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487715/
https://www.ncbi.nlm.nih.gov/pubmed/30803129
http://dx.doi.org/10.1002/1878-0261.12472
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