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Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II(®) CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia
This is the first report of QQQ-mass spectrometric identification and quantification of the Hb subunits, alpha, beta, delta and gamma globin peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the Bio-Rad cation-exchange chromatography of haemoglobin. The objectives...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488526/ https://www.ncbi.nlm.nih.gov/pubmed/31061897 http://dx.doi.org/10.1016/j.bbrep.2019.100635 |
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author | Abdullah, Uday Younis Hussein Ibrahim, Hishamshah M. Jassim, Haitham Muhammed Salleh, Mohamad Zaki Kek, Teh Lay Fakhruzzaman bin Noorizhab, Mohd Nur Zilfalil, Bin Alwi Wilairat, Prapin Fucharoen, Suthat |
author_facet | Abdullah, Uday Younis Hussein Ibrahim, Hishamshah M. Jassim, Haitham Muhammed Salleh, Mohamad Zaki Kek, Teh Lay Fakhruzzaman bin Noorizhab, Mohd Nur Zilfalil, Bin Alwi Wilairat, Prapin Fucharoen, Suthat |
author_sort | Abdullah, Uday Younis Hussein |
collection | PubMed |
description | This is the first report of QQQ-mass spectrometric identification and quantification of the Hb subunits, alpha, beta, delta and gamma globin peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the Bio-Rad cation-exchange chromatography of haemoglobin. The objectives were to assess the relationship of the quantity of the free alpha, beta, delta and gamma globin chains with the phenotypic diversity of beta-thalassaemias (β-thal). The results demonstrate that the pools of free globin chains in red blood cells were correlating with the severity of the disease in patients with different phenotypes of β-thal. The mechanism and the regulation of synthesis of free globin chains pool in a normal individual and in patients with different β-thal phenotypes could arise from several mechanisms which will require further investigation. The role of the free globin pool in patients with β-thal for development of novel therapeutic approaches based on these potential targets requires further investigation. Pertinent biomarkers improves the diagnosis of the β-thal, especially in low-income countries where they are most common and allows more effective therapeutic intervention leading to more successful therapeutic outcome. |
format | Online Article Text |
id | pubmed-6488526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-64885262019-05-06 Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II(®) CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia Abdullah, Uday Younis Hussein Ibrahim, Hishamshah M. Jassim, Haitham Muhammed Salleh, Mohamad Zaki Kek, Teh Lay Fakhruzzaman bin Noorizhab, Mohd Nur Zilfalil, Bin Alwi Wilairat, Prapin Fucharoen, Suthat Biochem Biophys Rep Research Article This is the first report of QQQ-mass spectrometric identification and quantification of the Hb subunits, alpha, beta, delta and gamma globin peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the Bio-Rad cation-exchange chromatography of haemoglobin. The objectives were to assess the relationship of the quantity of the free alpha, beta, delta and gamma globin chains with the phenotypic diversity of beta-thalassaemias (β-thal). The results demonstrate that the pools of free globin chains in red blood cells were correlating with the severity of the disease in patients with different phenotypes of β-thal. The mechanism and the regulation of synthesis of free globin chains pool in a normal individual and in patients with different β-thal phenotypes could arise from several mechanisms which will require further investigation. The role of the free globin pool in patients with β-thal for development of novel therapeutic approaches based on these potential targets requires further investigation. Pertinent biomarkers improves the diagnosis of the β-thal, especially in low-income countries where they are most common and allows more effective therapeutic intervention leading to more successful therapeutic outcome. Elsevier 2019-04-28 /pmc/articles/PMC6488526/ /pubmed/31061897 http://dx.doi.org/10.1016/j.bbrep.2019.100635 Text en © 2019 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Abdullah, Uday Younis Hussein Ibrahim, Hishamshah M. Jassim, Haitham Muhammed Salleh, Mohamad Zaki Kek, Teh Lay Fakhruzzaman bin Noorizhab, Mohd Nur Zilfalil, Bin Alwi Wilairat, Prapin Fucharoen, Suthat Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II(®) CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia |
title | Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II(®) CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia |
title_full | Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II(®) CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia |
title_fullStr | Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II(®) CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia |
title_full_unstemmed | Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II(®) CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia |
title_short | Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II(®) CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia |
title_sort | relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of bio-rad variant ii(®) ce-hplc of hemoglobin from healthy and beta-thalassemia subjects in malaysia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488526/ https://www.ncbi.nlm.nih.gov/pubmed/31061897 http://dx.doi.org/10.1016/j.bbrep.2019.100635 |
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