Development of a Blocking Primer to Inhibit the PCR Amplification of the 18S rDNA Sequences of Litopenaeus vannamei and Its Efficacy in Crassostrea hongkongensis

Diversity analyses of the eukaryotic microorganisms in the gut of marine animals is hampered by the presence of host DNA in the samples. PCR amplification of rRNA genes of eukaryotic microorganisms is inefficient with universal primers targeting 18S rRNA gene when the host DNA is dominant. In this s...

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Detalles Bibliográficos
Autores principales: Liu, Cong, Qi, Ren-Jie, Jiang, Jing-Zhe, Zhang, Ming-Qing, Wang, Jiang-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489566/
https://www.ncbi.nlm.nih.gov/pubmed/31065252
http://dx.doi.org/10.3389/fmicb.2019.00830

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489566/
https://www.ncbi.nlm.nih.gov/pubmed/31065252
http://dx.doi.org/10.3389/fmicb.2019.00830

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