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Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs

Here, we have established an antigen-specific single B cell sorting and monoclonal antibody (mAb) cloning platform for analyzing immunization- or viral infection-elicited antibody response at the clonal level in guinea pigs. We stained the peripheral blood mononuclear cells (PBMCs) from a guinea pig...

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Detalles Bibliográficos
Autores principales: Lei, Lin, Tran, Karen, Wang, Yimeng, Steinhardt, James J., Xiao, Yongli, Chiang, Chi-I, Wyatt, Richard T., Li, Yuxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489837/
https://www.ncbi.nlm.nih.gov/pubmed/31065249
http://dx.doi.org/10.3389/fmicb.2019.00672