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Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from Actinidia chinensis

BACKGROUND: Kiwifruit (Actinidia chinensis) peel has been always considered as useless because of the harsh taste. To promote the full utilization of kiwifruit resources it is essential to explore the nutritional benefits of kiwifruit peel. OBJECTIVE: Our studies explored the difference in polypheno...

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Autores principales: Alim, Aamina, Li, Ting, Nisar, Tanzeela, Ren, Daoyuan, Zhai, Xichuan, Pang, Yaxing, Yang, Xingbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Open Academia 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6495110/
https://www.ncbi.nlm.nih.gov/pubmed/31073285
http://dx.doi.org/10.29219/fnr.v63.1577
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author Alim, Aamina
Li, Ting
Nisar, Tanzeela
Ren, Daoyuan
Zhai, Xichuan
Pang, Yaxing
Yang, Xingbin
author_facet Alim, Aamina
Li, Ting
Nisar, Tanzeela
Ren, Daoyuan
Zhai, Xichuan
Pang, Yaxing
Yang, Xingbin
author_sort Alim, Aamina
collection PubMed
description BACKGROUND: Kiwifruit (Actinidia chinensis) peel has been always considered as useless because of the harsh taste. To promote the full utilization of kiwifruit resources it is essential to explore the nutritional benefits of kiwifruit peel. OBJECTIVE: Our studies explored the difference in polyphenolic composition and biological activity including antioxidant, antimicrobial, and antiproliferative activity of the flesh and peel of kiwifruit. DESIGN: Antioxidant activity of the extracted polyphenols of the peel and flesh of A. chinensis was checked by 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis3-ethylbenzothiazoline-6-sulphonic acid (ABTS), hydroxyl ion reduction, and ion chelating ability. Antibacterial activity against Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus and antiproliferative activity against HepG2 was tested in a dose- and time-dependent manner. Liquid chromatography/mass spectrometry (LC/MS) chromatogram of the peel and flesh further differentiated the phenolic acid profile. RESULTS: The pericarp of kiwifruit was found to be more abundant in polyphenols and flavonoids than the flesh, with contents of 12.8 mg/g and 2.7 mg/g, respectively. LC/MS analysis revealed that the catachin, quercetin and epigallocatechin content (the main polyphenols in kiwifruit) in the peel was significantly higher than in the flesh (P < 0.05). The antioxidant and antibacterial activity of the peel was significantly higher when compared to the flesh. Moreover, the proliferation of HepG2 cells was time- and dose-dependently inhibited by kiwifruit polyphenols, with IC(50) values of 170 μg/mL and 291 μg/mL for peel and flesh polyphenols after 72 h of treatment time, respectively. CONCLUSION: Kiwifruit peel, with higher content of phenolics and flavonoids, exerts more potent antioxidant, antibacterial, and anticancer activity than the flesh. Our study provides scientific evidence for the development of kiwifruit, especially peel-based, novel natural products with excellent bioactivity.
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spelling pubmed-64951102019-05-09 Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from Actinidia chinensis Alim, Aamina Li, Ting Nisar, Tanzeela Ren, Daoyuan Zhai, Xichuan Pang, Yaxing Yang, Xingbin Food Nutr Res Original Article BACKGROUND: Kiwifruit (Actinidia chinensis) peel has been always considered as useless because of the harsh taste. To promote the full utilization of kiwifruit resources it is essential to explore the nutritional benefits of kiwifruit peel. OBJECTIVE: Our studies explored the difference in polyphenolic composition and biological activity including antioxidant, antimicrobial, and antiproliferative activity of the flesh and peel of kiwifruit. DESIGN: Antioxidant activity of the extracted polyphenols of the peel and flesh of A. chinensis was checked by 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis3-ethylbenzothiazoline-6-sulphonic acid (ABTS), hydroxyl ion reduction, and ion chelating ability. Antibacterial activity against Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus and antiproliferative activity against HepG2 was tested in a dose- and time-dependent manner. Liquid chromatography/mass spectrometry (LC/MS) chromatogram of the peel and flesh further differentiated the phenolic acid profile. RESULTS: The pericarp of kiwifruit was found to be more abundant in polyphenols and flavonoids than the flesh, with contents of 12.8 mg/g and 2.7 mg/g, respectively. LC/MS analysis revealed that the catachin, quercetin and epigallocatechin content (the main polyphenols in kiwifruit) in the peel was significantly higher than in the flesh (P < 0.05). The antioxidant and antibacterial activity of the peel was significantly higher when compared to the flesh. Moreover, the proliferation of HepG2 cells was time- and dose-dependently inhibited by kiwifruit polyphenols, with IC(50) values of 170 μg/mL and 291 μg/mL for peel and flesh polyphenols after 72 h of treatment time, respectively. CONCLUSION: Kiwifruit peel, with higher content of phenolics and flavonoids, exerts more potent antioxidant, antibacterial, and anticancer activity than the flesh. Our study provides scientific evidence for the development of kiwifruit, especially peel-based, novel natural products with excellent bioactivity. Open Academia 2019-04-26 /pmc/articles/PMC6495110/ /pubmed/31073285 http://dx.doi.org/10.29219/fnr.v63.1577 Text en © 2019 Aamina Alim et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
spellingShingle Original Article
Alim, Aamina
Li, Ting
Nisar, Tanzeela
Ren, Daoyuan
Zhai, Xichuan
Pang, Yaxing
Yang, Xingbin
Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from Actinidia chinensis
title Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from Actinidia chinensis
title_full Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from Actinidia chinensis
title_fullStr Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from Actinidia chinensis
title_full_unstemmed Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from Actinidia chinensis
title_short Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from Actinidia chinensis
title_sort antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from actinidia chinensis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6495110/
https://www.ncbi.nlm.nih.gov/pubmed/31073285
http://dx.doi.org/10.29219/fnr.v63.1577
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