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A simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells

We introduce a simple and universal cloning plasmid system for gene expression in prokaryotic (Escherichia coli) and mammalian cells. This novel system has two expression modes: the (subcloning) prokaryotic and mammalian modes. This system streamlines the process of producing mammalian gene expressi...

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Detalles Bibliográficos
Autores principales: Murakami, Manabu, Ohba, Takayoshi, Murakami, Agnieszka M., Han, Chong, Kuwasako, Kenji, Itagaki, Shirou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6497378/
https://www.ncbi.nlm.nih.gov/pubmed/31048860
http://dx.doi.org/10.1371/journal.pone.0216169