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A simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells
We introduce a simple and universal cloning plasmid system for gene expression in prokaryotic (Escherichia coli) and mammalian cells. This novel system has two expression modes: the (subcloning) prokaryotic and mammalian modes. This system streamlines the process of producing mammalian gene expressi...
Autores principales: | Murakami, Manabu, Ohba, Takayoshi, Murakami, Agnieszka M., Han, Chong, Kuwasako, Kenji, Itagaki, Shirou |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6497378/ https://www.ncbi.nlm.nih.gov/pubmed/31048860 http://dx.doi.org/10.1371/journal.pone.0216169 |
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