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Use of Plasmid pVMG to Make Transcriptional ß-Glucuronidase Reporter Gene Fusions in the Rhizobium Genome for Monitoring the Expression of Rhizobial Genes In Vivo

BACKGROUND: The soil bacterium Sinorhizobium meliloti and its allies are important nitrogen-fixing bacterial symbionts that cause N(2)-fixing nodules on the roots of legumes. Chromosomal ß-glucuronidase gene (uidA) transcriptional fusions are frequently used to monitor the expression of bacterial ge...

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Detalles Bibliográficos
Autores principales: Gao, Mengsheng, Benge, Anne, Wu, Tai-Jung, Javier, Regina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498626/
https://www.ncbi.nlm.nih.gov/pubmed/31073281
http://dx.doi.org/10.1186/s12575-019-0096-y