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Use of Plasmid pVMG to Make Transcriptional ß-Glucuronidase Reporter Gene Fusions in the Rhizobium Genome for Monitoring the Expression of Rhizobial Genes In Vivo

BACKGROUND: The soil bacterium Sinorhizobium meliloti and its allies are important nitrogen-fixing bacterial symbionts that cause N(2)-fixing nodules on the roots of legumes. Chromosomal ß-glucuronidase gene (uidA) transcriptional fusions are frequently used to monitor the expression of bacterial ge...

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Detalles Bibliográficos
Autores principales:	Gao, Mengsheng, Benge, Anne, Wu, Tai-Jung, Javier, Regina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498626/ https://www.ncbi.nlm.nih.gov/pubmed/31073281 http://dx.doi.org/10.1186/s12575-019-0096-y

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