Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins

CRISPR/Cas9 ribonucleoprotein (RNP) complexes are promising biological tools with diverse biomedical applications. However, to date there are no efficient methods that can produce these proteins at large scales and low cost. Here, we present a streamlined method for direct production of Cas9 RNPs fr...

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Detalles Bibliográficos
Autores principales: Qiao, Jie, Li, Wenqiang, Lin, Siyu, Sun, Wenli, Ma, Lixin, Liu, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499778/
https://www.ncbi.nlm.nih.gov/pubmed/31069270
http://dx.doi.org/10.1038/s42003-019-0402-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499778/
https://www.ncbi.nlm.nih.gov/pubmed/31069270
http://dx.doi.org/10.1038/s42003-019-0402-x

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