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Nanopore sequencing of long ribosomal DNA amplicons enables portable and simple biodiversity assessments with high phylogenetic resolution across broad taxonomic scale

BACKGROUND: In light of the current biodiversity crisis, DNA barcoding is developing into an essential tool to quantify state shifts in global ecosystems. Current barcoding protocols often rely on short amplicon sequences, which yield accurate identification of biological entities in a community but...

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Detalles Bibliográficos
Autores principales: Krehenwinkel, Henrik, Pomerantz, Aaron, Henderson, James B, Kennedy, Susan R, Lim, Jun Ying, Swamy, Varun, Shoobridge, Juan Diego, Graham, Natalie, Patel, Nipam H, Gillespie, Rosemary G, Prost, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503943/
https://www.ncbi.nlm.nih.gov/pubmed/30824940
http://dx.doi.org/10.1093/gigascience/giz006