Cargando…
A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis
During infection, pathogens are starved of essential nutrients such as iron and tryptophan by host immune effectors. Without conserved global stress response regulators, how the obligate intracellular bacterium Chlamydia trachomatis arrives at a physiologically similar ‘persistent’ state in response...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504234/ https://www.ncbi.nlm.nih.gov/pubmed/30938288 http://dx.doi.org/10.7554/eLife.42295 |
_version_ | 1783416533517598720 |
---|---|
author | Pokorzynski, Nick D Brinkworth, Amanda J Carabeo, Rey |
author_facet | Pokorzynski, Nick D Brinkworth, Amanda J Carabeo, Rey |
author_sort | Pokorzynski, Nick D |
collection | PubMed |
description | During infection, pathogens are starved of essential nutrients such as iron and tryptophan by host immune effectors. Without conserved global stress response regulators, how the obligate intracellular bacterium Chlamydia trachomatis arrives at a physiologically similar ‘persistent’ state in response to starvation of either nutrient remains unclear. Here, we report on the iron-dependent regulation of the trpRBA tryptophan salvage pathway in C. trachomatis. Iron starvation specifically induces trpBA expression from a novel promoter element within an intergenic region flanked by trpR and trpB. YtgR, the only known iron-dependent regulator in Chlamydia, can bind to the trpRBA intergenic region upstream of the alternative trpBA promoter to repress transcription. Simultaneously, YtgR binding promotes the termination of transcripts from the primary promoter upstream of trpR. This is the first description of an iron-dependent mechanism regulating prokaryotic tryptophan biosynthesis that may indicate the existence of novel approaches to gene regulation and stress response in Chlamydia. |
format | Online Article Text |
id | pubmed-6504234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65042342019-05-09 A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis Pokorzynski, Nick D Brinkworth, Amanda J Carabeo, Rey eLife Chromosomes and Gene Expression During infection, pathogens are starved of essential nutrients such as iron and tryptophan by host immune effectors. Without conserved global stress response regulators, how the obligate intracellular bacterium Chlamydia trachomatis arrives at a physiologically similar ‘persistent’ state in response to starvation of either nutrient remains unclear. Here, we report on the iron-dependent regulation of the trpRBA tryptophan salvage pathway in C. trachomatis. Iron starvation specifically induces trpBA expression from a novel promoter element within an intergenic region flanked by trpR and trpB. YtgR, the only known iron-dependent regulator in Chlamydia, can bind to the trpRBA intergenic region upstream of the alternative trpBA promoter to repress transcription. Simultaneously, YtgR binding promotes the termination of transcripts from the primary promoter upstream of trpR. This is the first description of an iron-dependent mechanism regulating prokaryotic tryptophan biosynthesis that may indicate the existence of novel approaches to gene regulation and stress response in Chlamydia. eLife Sciences Publications, Ltd 2019-04-02 /pmc/articles/PMC6504234/ /pubmed/30938288 http://dx.doi.org/10.7554/eLife.42295 Text en © 2019, Pokorzynski et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Chromosomes and Gene Expression Pokorzynski, Nick D Brinkworth, Amanda J Carabeo, Rey A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis |
title | A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis |
title_full | A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis |
title_fullStr | A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis |
title_full_unstemmed | A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis |
title_short | A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in Chlamydia trachomatis |
title_sort | bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway in chlamydia trachomatis |
topic | Chromosomes and Gene Expression |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504234/ https://www.ncbi.nlm.nih.gov/pubmed/30938288 http://dx.doi.org/10.7554/eLife.42295 |
work_keys_str_mv | AT pokorzynskinickd abipartiteirondependenttranscriptionalregulationofthetryptophansalvagepathwayinchlamydiatrachomatis AT brinkworthamandaj abipartiteirondependenttranscriptionalregulationofthetryptophansalvagepathwayinchlamydiatrachomatis AT carabeorey abipartiteirondependenttranscriptionalregulationofthetryptophansalvagepathwayinchlamydiatrachomatis AT pokorzynskinickd bipartiteirondependenttranscriptionalregulationofthetryptophansalvagepathwayinchlamydiatrachomatis AT brinkworthamandaj bipartiteirondependenttranscriptionalregulationofthetryptophansalvagepathwayinchlamydiatrachomatis AT carabeorey bipartiteirondependenttranscriptionalregulationofthetryptophansalvagepathwayinchlamydiatrachomatis |