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Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production

BACKGROUND: Bacteria are widely used as hosts for recombinant protein production due to their rapid growth, simple media requirement and ability to produce high yields of correctly folded proteins. Overproduction of recombinant proteins may impose metabolic burden to host cells, triggering various s...

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Detalles Bibliográficos
Autores principales: Gawin, Agnieszka, Peebo, Karl, Hans, Sebastian, Ertesvåg, Helga, Irla, Marta, Neubauer, Peter, Brautaset, Trygve
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505264/
https://www.ncbi.nlm.nih.gov/pubmed/31064376
http://dx.doi.org/10.1186/s12934-019-1128-7