Live pigs produced from genome edited zygotes

Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Mono...

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Autores principales: Lillico, Simon G., Proudfoot, Chris, Carlson, Daniel F., Stverakova, Dana, Neil, Claire, Blain, Carol, King, Tim J., Ritchie, William A., Tan, Wenfang, Mileham, Alan J., McLaren, David G., Fahrenkrug, Scott C., Whitelaw, C. Bruce A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505673/
https://www.ncbi.nlm.nih.gov/pubmed/24108318
http://dx.doi.org/10.1038/srep02847
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author Lillico, Simon G.
Proudfoot, Chris
Carlson, Daniel F.
Stverakova, Dana
Neil, Claire
Blain, Carol
King, Tim J.
Ritchie, William A.
Tan, Wenfang
Mileham, Alan J.
McLaren, David G.
Fahrenkrug, Scott C.
Whitelaw, C. Bruce A.
author_facet Lillico, Simon G.
Proudfoot, Chris
Carlson, Daniel F.
Stverakova, Dana
Neil, Claire
Blain, Carol
King, Tim J.
Ritchie, William A.
Tan, Wenfang
Mileham, Alan J.
McLaren, David G.
Fahrenkrug, Scott C.
Whitelaw, C. Bruce A.
author_sort Lillico, Simon G.
collection PubMed
description Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.
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spelling pubmed-65056732019-05-21 Live pigs produced from genome edited zygotes Lillico, Simon G. Proudfoot, Chris Carlson, Daniel F. Stverakova, Dana Neil, Claire Blain, Carol King, Tim J. Ritchie, William A. Tan, Wenfang Mileham, Alan J. McLaren, David G. Fahrenkrug, Scott C. Whitelaw, C. Bruce A. Sci Rep Article Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating. Nature Publishing Group 2013-10-10 /pmc/articles/PMC6505673/ /pubmed/24108318 http://dx.doi.org/10.1038/srep02847 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Lillico, Simon G.
Proudfoot, Chris
Carlson, Daniel F.
Stverakova, Dana
Neil, Claire
Blain, Carol
King, Tim J.
Ritchie, William A.
Tan, Wenfang
Mileham, Alan J.
McLaren, David G.
Fahrenkrug, Scott C.
Whitelaw, C. Bruce A.
Live pigs produced from genome edited zygotes
title Live pigs produced from genome edited zygotes
title_full Live pigs produced from genome edited zygotes
title_fullStr Live pigs produced from genome edited zygotes
title_full_unstemmed Live pigs produced from genome edited zygotes
title_short Live pigs produced from genome edited zygotes
title_sort live pigs produced from genome edited zygotes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505673/
https://www.ncbi.nlm.nih.gov/pubmed/24108318
http://dx.doi.org/10.1038/srep02847
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