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A strain comparison of Campylobacter isolated from retail poultry and human clinical cases in Atlantic Canada

Campylobacter is the leading cause of food-borne bacterial disease in Canada and many developed countries. One of the most common sources of human campylobacteriosis is considered to be the consumption or handling of raw or undercooked poultry. To date, few Canadian studies have investigated both th...

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Autores principales: Hodges, Lisa M., Carrillo, Catherine D., Upham, Jacqueline P., Borza, Antonela, Eisebraun, Mikaela, Kenwell, Robyn, Mutschall, Steven K., Haldane, David, Schleihauf, Emily, Taboada, Eduardo N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505856/
https://www.ncbi.nlm.nih.gov/pubmed/31067242
http://dx.doi.org/10.1371/journal.pone.0215928
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author Hodges, Lisa M.
Carrillo, Catherine D.
Upham, Jacqueline P.
Borza, Antonela
Eisebraun, Mikaela
Kenwell, Robyn
Mutschall, Steven K.
Haldane, David
Schleihauf, Emily
Taboada, Eduardo N.
author_facet Hodges, Lisa M.
Carrillo, Catherine D.
Upham, Jacqueline P.
Borza, Antonela
Eisebraun, Mikaela
Kenwell, Robyn
Mutschall, Steven K.
Haldane, David
Schleihauf, Emily
Taboada, Eduardo N.
author_sort Hodges, Lisa M.
collection PubMed
description Campylobacter is the leading cause of food-borne bacterial disease in Canada and many developed countries. One of the most common sources of human campylobacteriosis is considered to be the consumption or handling of raw or undercooked poultry. To date, few Canadian studies have investigated both the prevalence of Campylobacter on retail poultry and its potential impact on human clinical cases. The objective of this study was to evaluate the prevalence of Campylobacter spp. at the retail level and the correlation between subtypes recovered from chicken and those recovered from human clinical cases within the province of Nova Scotia, Canada. From this study 354 human clinical isolates were obtained from provincial hospital laboratories and a total of 480 packages of raw poultry cuts were sampled from retail outlets, yielding 312 isolates (65%), of all which were subtyped using comparative genomic fingerprinting (CGF). Of the 312 chicken isolates, the majority of isolates were C. jejuni (91.7%), followed by C. coli (7.7%) and C. lari (0.6%). Using CGF to subtype C. jejuni and C. coli isolates, 99 and 152 subtypes were recovered from chicken and clinical cases, respectively. The most prevalent human and chicken subtypes found in NS are similar to those observed nationally; indicating that the Campylobacter from this study appear to reflect of the profile of Campylobacter subtypes circulating nationally. Of the subtypes observed, only 36 subtypes were common between the two groups, however, these subtypes represented 48.3% of the clinical isolates collected. The findings from this study provides evidence that in Nova Scotia, retail poultry can act as a reservoir for Campylobacter subtypes that have been implicated in human illness.
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spelling pubmed-65058562019-05-23 A strain comparison of Campylobacter isolated from retail poultry and human clinical cases in Atlantic Canada Hodges, Lisa M. Carrillo, Catherine D. Upham, Jacqueline P. Borza, Antonela Eisebraun, Mikaela Kenwell, Robyn Mutschall, Steven K. Haldane, David Schleihauf, Emily Taboada, Eduardo N. PLoS One Research Article Campylobacter is the leading cause of food-borne bacterial disease in Canada and many developed countries. One of the most common sources of human campylobacteriosis is considered to be the consumption or handling of raw or undercooked poultry. To date, few Canadian studies have investigated both the prevalence of Campylobacter on retail poultry and its potential impact on human clinical cases. The objective of this study was to evaluate the prevalence of Campylobacter spp. at the retail level and the correlation between subtypes recovered from chicken and those recovered from human clinical cases within the province of Nova Scotia, Canada. From this study 354 human clinical isolates were obtained from provincial hospital laboratories and a total of 480 packages of raw poultry cuts were sampled from retail outlets, yielding 312 isolates (65%), of all which were subtyped using comparative genomic fingerprinting (CGF). Of the 312 chicken isolates, the majority of isolates were C. jejuni (91.7%), followed by C. coli (7.7%) and C. lari (0.6%). Using CGF to subtype C. jejuni and C. coli isolates, 99 and 152 subtypes were recovered from chicken and clinical cases, respectively. The most prevalent human and chicken subtypes found in NS are similar to those observed nationally; indicating that the Campylobacter from this study appear to reflect of the profile of Campylobacter subtypes circulating nationally. Of the subtypes observed, only 36 subtypes were common between the two groups, however, these subtypes represented 48.3% of the clinical isolates collected. The findings from this study provides evidence that in Nova Scotia, retail poultry can act as a reservoir for Campylobacter subtypes that have been implicated in human illness. Public Library of Science 2019-05-08 /pmc/articles/PMC6505856/ /pubmed/31067242 http://dx.doi.org/10.1371/journal.pone.0215928 Text en © 2019 Hodges et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hodges, Lisa M.
Carrillo, Catherine D.
Upham, Jacqueline P.
Borza, Antonela
Eisebraun, Mikaela
Kenwell, Robyn
Mutschall, Steven K.
Haldane, David
Schleihauf, Emily
Taboada, Eduardo N.
A strain comparison of Campylobacter isolated from retail poultry and human clinical cases in Atlantic Canada
title A strain comparison of Campylobacter isolated from retail poultry and human clinical cases in Atlantic Canada
title_full A strain comparison of Campylobacter isolated from retail poultry and human clinical cases in Atlantic Canada
title_fullStr A strain comparison of Campylobacter isolated from retail poultry and human clinical cases in Atlantic Canada
title_full_unstemmed A strain comparison of Campylobacter isolated from retail poultry and human clinical cases in Atlantic Canada
title_short A strain comparison of Campylobacter isolated from retail poultry and human clinical cases in Atlantic Canada
title_sort strain comparison of campylobacter isolated from retail poultry and human clinical cases in atlantic canada
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505856/
https://www.ncbi.nlm.nih.gov/pubmed/31067242
http://dx.doi.org/10.1371/journal.pone.0215928
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