Methods toward simplification of time resolved fluorescence anisotropy in proteins labeled with NBD (4-chloro-7-nitrobenzofurazan) adducts

The analysis of time resolved fluorescence anisotropy for NBD tagged proteins is difficult when multiple exponential components arise from heterogeneous amino acid fluorescent adducts. Two approaches were taken toward simplification. First, N terminal selective labeling of tear lipocalin with NBD-Cl...

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Detalles Bibliográficos
Autores principales: Glasgow, Ben J., Abduragimov, Adil R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506467/
https://www.ncbi.nlm.nih.gov/pubmed/31080764
http://dx.doi.org/10.1016/j.mex.2019.04.025

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506467/
https://www.ncbi.nlm.nih.gov/pubmed/31080764
http://dx.doi.org/10.1016/j.mex.2019.04.025

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