Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in Pseudomonas putida Lysine Metabolism

Despite intensive study for 50 years, the biochemical and genetic links between lysine metabolism and central metabolism in Pseudomonas putida remain unresolved. To establish these biochemical links, we leveraged random barcode transposon sequencing (RB-TnSeq), a genome-wide assay measuring the fitn...

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Detalles Bibliográficos
Autores principales: Thompson, Mitchell G., Blake-Hedges, Jacquelyn M., Cruz-Morales, Pablo, Barajas, Jesus F., Curran, Samuel C., Eiben, Christopher B., Harris, Nicholas C., Benites, Veronica T., Gin, Jennifer W., Sharpless, William A., Twigg, Frederick F., Skyrud, Will, Krishna, Rohith N., Pereira, Jose Henrique, Baidoo, Edward E. K., Petzold, Christopher J., Adams, Paul D., Arkin, Adam P., Deutschbauer, Adam M., Keasling, Jay D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509195/
https://www.ncbi.nlm.nih.gov/pubmed/31064836
http://dx.doi.org/10.1128/mBio.02577-18

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509195/
https://www.ncbi.nlm.nih.gov/pubmed/31064836
http://dx.doi.org/10.1128/mBio.02577-18

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