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Ageing characteristics of bone indicated by transcriptomic and exosomal proteomic analysis of cortical bone cells

BACKGROUND: Degenerative changes in the skeleton play an important role in ageing. As the foremost sensors and orchestrators of bone remodelling, osteocytes contribute significantly to the health of the skeleton. Embedded in a mineralized bone matrix, the osteocyte network and the surrounding lacuna...

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Autores principales: Zhang, Chenyang, Xu, Shuyu, Zhang, Shufan, Liu, Mengmeng, Du, Haiming, Sun, Ruinan, Jing, Bo, Sun, Yao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509863/
https://www.ncbi.nlm.nih.gov/pubmed/31077243
http://dx.doi.org/10.1186/s13018-019-1163-4
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author Zhang, Chenyang
Xu, Shuyu
Zhang, Shufan
Liu, Mengmeng
Du, Haiming
Sun, Ruinan
Jing, Bo
Sun, Yao
author_facet Zhang, Chenyang
Xu, Shuyu
Zhang, Shufan
Liu, Mengmeng
Du, Haiming
Sun, Ruinan
Jing, Bo
Sun, Yao
author_sort Zhang, Chenyang
collection PubMed
description BACKGROUND: Degenerative changes in the skeleton play an important role in ageing. As the foremost sensors and orchestrators of bone remodelling, osteocytes contribute significantly to the health of the skeleton. Embedded in a mineralized bone matrix, the osteocyte network and the surrounding lacunar canaliculae work together as a functional syncytium—the osteocytic lacunar-canalicular system (OLCS). However, changes in the OLCS during ageing and related mechanisms cannot be fully understood by using traditional histological analysis. METHODS: To link the phenotypes of aged osteocytes and their functional changes during ageing, we analysed the changes in the gene expression profiles of bone cells and the proteomic profiles of OLCS exosomes derived from aged and young cortical bone. RESULTS: Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes (DEGs) suggested that a decline in cell energy metabolism and an increased level of the proinflammatory state are major characteristics of bone ageing. Moreover, some DEGs were key regulators of bone mechanical sensation and bone remodelling, which are indicative of reduced bone-specific function with age. Further, the identified proteins in OLCS exosomes showed potential changes in the secretory function bone. Compared with young controls, the decreased functional proteins in aged OLCS exosomes were enriched mainly in GO terms that included regulating bone development and remodelling, cell-matrix adhesion, and cell clearance and homeostasis. Notably, several functions of exosomal proteins of the aged group revealed potential new roles, such as regulating innate and adaptive immunity, wound healing, and angiogenesis and eliminating oxidative stress. CONCLUSION: The information obtained from bone cells and OLCS exosomes will help us discover new features of bone ageing. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13018-019-1163-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-65098632019-06-05 Ageing characteristics of bone indicated by transcriptomic and exosomal proteomic analysis of cortical bone cells Zhang, Chenyang Xu, Shuyu Zhang, Shufan Liu, Mengmeng Du, Haiming Sun, Ruinan Jing, Bo Sun, Yao J Orthop Surg Res Research Article BACKGROUND: Degenerative changes in the skeleton play an important role in ageing. As the foremost sensors and orchestrators of bone remodelling, osteocytes contribute significantly to the health of the skeleton. Embedded in a mineralized bone matrix, the osteocyte network and the surrounding lacunar canaliculae work together as a functional syncytium—the osteocytic lacunar-canalicular system (OLCS). However, changes in the OLCS during ageing and related mechanisms cannot be fully understood by using traditional histological analysis. METHODS: To link the phenotypes of aged osteocytes and their functional changes during ageing, we analysed the changes in the gene expression profiles of bone cells and the proteomic profiles of OLCS exosomes derived from aged and young cortical bone. RESULTS: Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes (DEGs) suggested that a decline in cell energy metabolism and an increased level of the proinflammatory state are major characteristics of bone ageing. Moreover, some DEGs were key regulators of bone mechanical sensation and bone remodelling, which are indicative of reduced bone-specific function with age. Further, the identified proteins in OLCS exosomes showed potential changes in the secretory function bone. Compared with young controls, the decreased functional proteins in aged OLCS exosomes were enriched mainly in GO terms that included regulating bone development and remodelling, cell-matrix adhesion, and cell clearance and homeostasis. Notably, several functions of exosomal proteins of the aged group revealed potential new roles, such as regulating innate and adaptive immunity, wound healing, and angiogenesis and eliminating oxidative stress. CONCLUSION: The information obtained from bone cells and OLCS exosomes will help us discover new features of bone ageing. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13018-019-1163-4) contains supplementary material, which is available to authorized users. BioMed Central 2019-05-10 /pmc/articles/PMC6509863/ /pubmed/31077243 http://dx.doi.org/10.1186/s13018-019-1163-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Chenyang
Xu, Shuyu
Zhang, Shufan
Liu, Mengmeng
Du, Haiming
Sun, Ruinan
Jing, Bo
Sun, Yao
Ageing characteristics of bone indicated by transcriptomic and exosomal proteomic analysis of cortical bone cells
title Ageing characteristics of bone indicated by transcriptomic and exosomal proteomic analysis of cortical bone cells
title_full Ageing characteristics of bone indicated by transcriptomic and exosomal proteomic analysis of cortical bone cells
title_fullStr Ageing characteristics of bone indicated by transcriptomic and exosomal proteomic analysis of cortical bone cells
title_full_unstemmed Ageing characteristics of bone indicated by transcriptomic and exosomal proteomic analysis of cortical bone cells
title_short Ageing characteristics of bone indicated by transcriptomic and exosomal proteomic analysis of cortical bone cells
title_sort ageing characteristics of bone indicated by transcriptomic and exosomal proteomic analysis of cortical bone cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509863/
https://www.ncbi.nlm.nih.gov/pubmed/31077243
http://dx.doi.org/10.1186/s13018-019-1163-4
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