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Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species

Proteasome inhibition represents an important anticancer strategy. Here, we studied the mechanisms at the basis of the pro-apoptotic activity of the standardized decoction of Hemidesmus indicus, a plant evoking a complex anticancer activity, and explored its inhibition of proteasome activity in huma...

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Detalles Bibliográficos
Autores principales: Turrini, Eleonora, Catanzaro, Elena, Ferruzzi, Lorenzo, Guerrini, Alessandra, Tacchini, Massimo, Sacchetti, Gianni, Paganetto, Guglielmo, Maffei, Francesca, Pellicioni, Valentina, Poli, Ferruccio, Hrelia, Patrizia, Mandrone, Manuela, Sestili, Piero, Brigotti, Maurizio, Fimognari, Carmela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6510901/
https://www.ncbi.nlm.nih.gov/pubmed/31076590
http://dx.doi.org/10.1038/s41598-019-43609-5
Descripción
Sumario:Proteasome inhibition represents an important anticancer strategy. Here, we studied the mechanisms at the basis of the pro-apoptotic activity of the standardized decoction of Hemidesmus indicus, a plant evoking a complex anticancer activity, and explored its inhibition of proteasome activity in human leukemia cells. Additionally, we preliminary tested the cytotoxicity of some H. indicus’s phytochemicals on leukemia cells and their intestinal absorption on a human intestinal epithelium model consisting of a monolayer of differentiated Caco2 cells. We observed a potent antileukemic effect for H. indicus, imputable to the modulation of different critical targets at protein and mRNA levels and the reduction of the 26S proteasome expression. We found that some phytomarkers of H. indicus decoction passed through the enterocyte monolayer. Overall, our study supports the pharmacological potential of H. indicus, which can represent an interesting botanical drug in the oncological area.