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Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species

Proteasome inhibition represents an important anticancer strategy. Here, we studied the mechanisms at the basis of the pro-apoptotic activity of the standardized decoction of Hemidesmus indicus, a plant evoking a complex anticancer activity, and explored its inhibition of proteasome activity in huma...

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Autores principales: Turrini, Eleonora, Catanzaro, Elena, Ferruzzi, Lorenzo, Guerrini, Alessandra, Tacchini, Massimo, Sacchetti, Gianni, Paganetto, Guglielmo, Maffei, Francesca, Pellicioni, Valentina, Poli, Ferruccio, Hrelia, Patrizia, Mandrone, Manuela, Sestili, Piero, Brigotti, Maurizio, Fimognari, Carmela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6510901/
https://www.ncbi.nlm.nih.gov/pubmed/31076590
http://dx.doi.org/10.1038/s41598-019-43609-5
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author Turrini, Eleonora
Catanzaro, Elena
Ferruzzi, Lorenzo
Guerrini, Alessandra
Tacchini, Massimo
Sacchetti, Gianni
Paganetto, Guglielmo
Maffei, Francesca
Pellicioni, Valentina
Poli, Ferruccio
Hrelia, Patrizia
Mandrone, Manuela
Sestili, Piero
Brigotti, Maurizio
Fimognari, Carmela
author_facet Turrini, Eleonora
Catanzaro, Elena
Ferruzzi, Lorenzo
Guerrini, Alessandra
Tacchini, Massimo
Sacchetti, Gianni
Paganetto, Guglielmo
Maffei, Francesca
Pellicioni, Valentina
Poli, Ferruccio
Hrelia, Patrizia
Mandrone, Manuela
Sestili, Piero
Brigotti, Maurizio
Fimognari, Carmela
author_sort Turrini, Eleonora
collection PubMed
description Proteasome inhibition represents an important anticancer strategy. Here, we studied the mechanisms at the basis of the pro-apoptotic activity of the standardized decoction of Hemidesmus indicus, a plant evoking a complex anticancer activity, and explored its inhibition of proteasome activity in human leukemia cells. Additionally, we preliminary tested the cytotoxicity of some H. indicus’s phytochemicals on leukemia cells and their intestinal absorption on a human intestinal epithelium model consisting of a monolayer of differentiated Caco2 cells. We observed a potent antileukemic effect for H. indicus, imputable to the modulation of different critical targets at protein and mRNA levels and the reduction of the 26S proteasome expression. We found that some phytomarkers of H. indicus decoction passed through the enterocyte monolayer. Overall, our study supports the pharmacological potential of H. indicus, which can represent an interesting botanical drug in the oncological area.
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spelling pubmed-65109012019-05-23 Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species Turrini, Eleonora Catanzaro, Elena Ferruzzi, Lorenzo Guerrini, Alessandra Tacchini, Massimo Sacchetti, Gianni Paganetto, Guglielmo Maffei, Francesca Pellicioni, Valentina Poli, Ferruccio Hrelia, Patrizia Mandrone, Manuela Sestili, Piero Brigotti, Maurizio Fimognari, Carmela Sci Rep Article Proteasome inhibition represents an important anticancer strategy. Here, we studied the mechanisms at the basis of the pro-apoptotic activity of the standardized decoction of Hemidesmus indicus, a plant evoking a complex anticancer activity, and explored its inhibition of proteasome activity in human leukemia cells. Additionally, we preliminary tested the cytotoxicity of some H. indicus’s phytochemicals on leukemia cells and their intestinal absorption on a human intestinal epithelium model consisting of a monolayer of differentiated Caco2 cells. We observed a potent antileukemic effect for H. indicus, imputable to the modulation of different critical targets at protein and mRNA levels and the reduction of the 26S proteasome expression. We found that some phytomarkers of H. indicus decoction passed through the enterocyte monolayer. Overall, our study supports the pharmacological potential of H. indicus, which can represent an interesting botanical drug in the oncological area. Nature Publishing Group UK 2019-05-10 /pmc/articles/PMC6510901/ /pubmed/31076590 http://dx.doi.org/10.1038/s41598-019-43609-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Turrini, Eleonora
Catanzaro, Elena
Ferruzzi, Lorenzo
Guerrini, Alessandra
Tacchini, Massimo
Sacchetti, Gianni
Paganetto, Guglielmo
Maffei, Francesca
Pellicioni, Valentina
Poli, Ferruccio
Hrelia, Patrizia
Mandrone, Manuela
Sestili, Piero
Brigotti, Maurizio
Fimognari, Carmela
Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species
title Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species
title_full Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species
title_fullStr Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species
title_full_unstemmed Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species
title_short Hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species
title_sort hemidesmus indicus induces apoptosis via proteasome inhibition and generation of reactive oxygen species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6510901/
https://www.ncbi.nlm.nih.gov/pubmed/31076590
http://dx.doi.org/10.1038/s41598-019-43609-5
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