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ClampFISH detects individual nucleic-acid molecules using click chemistry based amplification
Methods for detecting single nucleic acids in cell and tissues, such as fluorescence in situ hybridization (FISH), are limited by relatively low signal intensity and non-specific probe binding. Here we present click-amplifying FISH (clampFISH), a method for fluorescence detection of nucleic acids th...
Autores principales: | Rouhanifard, Sara H., Mellis, Ian A., Dunagin, Margaret, Bayatpour, Sareh, Jiang, Connie L., Dardani, Ian, Symmons, Orsolya, Emert, Benjamin, Torre, Eduardo, Cote, Allison, Sullivan, Alessandra, Stamatoyannopoulos, John A., Raj, Arjun |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511493/ https://www.ncbi.nlm.nih.gov/pubmed/30418432 http://dx.doi.org/10.1038/nbt.4286 |
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