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Efficient Stereospecific H(β2/3) NMR Assignment Strategy for Mid-Size Proteins

We present a strategy for stereospecific NMR assignment of H(β2) and H(β3) protons in mid-size proteins (~150 residues). For such proteins, resonance overlap in standard experiments is severe, thereby preventing unambiguous assignment of a large fraction of β-methylenes. To alleviate this limitation...

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Detalles Bibliográficos
Autores principales: Born, Alexandra, Henen, Morkos A., Nichols, Parker, Wang, Jing, Jones, David N., Vögeli, Beat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6513325/
https://www.ncbi.nlm.nih.gov/pubmed/31093488
http://dx.doi.org/10.3390/magnetochemistry4020025