Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes
Two near-infrared fluorescent probes (A and B) containing hemicyanine structures appended to dipicolylamine (DPA), and a dipicolylamine derivative where one pyridine was substituted with pyrazine, respectively, were synthesized and tested for the identification of Zn(II) ions in live cells. In both...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515227/ https://www.ncbi.nlm.nih.gov/pubmed/31013675 http://dx.doi.org/10.3390/molecules24081592 |
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author | Fang, Mingxi Xia, Shuai Bi, Jianheng Wigstrom, Travis P. Valenzano, Loredana Wang, Jianbo Tanasova, Marina Luck, Rudy L. Liu, Haiying |
author_facet | Fang, Mingxi Xia, Shuai Bi, Jianheng Wigstrom, Travis P. Valenzano, Loredana Wang, Jianbo Tanasova, Marina Luck, Rudy L. Liu, Haiying |
author_sort | Fang, Mingxi |
collection | PubMed |
description | Two near-infrared fluorescent probes (A and B) containing hemicyanine structures appended to dipicolylamine (DPA), and a dipicolylamine derivative where one pyridine was substituted with pyrazine, respectively, were synthesized and tested for the identification of Zn(II) ions in live cells. In both probes, an acetyl group is attached to the phenolic oxygen atom of the hemicyanine platform to decrease the probe fluorescence background. Probe A displays sensitive fluorescence responses and binds preferentially to Zn(II) ions over other metal ions such as Cd(2+) ions with a low detection limit of 0.45 nM. In contrast, the emission spectra of probe B is not significantly affected if Zn(II) ions are added. Probe A possesses excellent membrane permeability and low cytotoxicity, allowing for sensitive imaging of both exogenously supplemented Zn(II) ions in live cells, and endogenously releases Zn(II) ions in cells after treatment of 2,2-dithiodipyridine. |
format | Online Article Text |
id | pubmed-6515227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65152272019-05-30 Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes Fang, Mingxi Xia, Shuai Bi, Jianheng Wigstrom, Travis P. Valenzano, Loredana Wang, Jianbo Tanasova, Marina Luck, Rudy L. Liu, Haiying Molecules Article Two near-infrared fluorescent probes (A and B) containing hemicyanine structures appended to dipicolylamine (DPA), and a dipicolylamine derivative where one pyridine was substituted with pyrazine, respectively, were synthesized and tested for the identification of Zn(II) ions in live cells. In both probes, an acetyl group is attached to the phenolic oxygen atom of the hemicyanine platform to decrease the probe fluorescence background. Probe A displays sensitive fluorescence responses and binds preferentially to Zn(II) ions over other metal ions such as Cd(2+) ions with a low detection limit of 0.45 nM. In contrast, the emission spectra of probe B is not significantly affected if Zn(II) ions are added. Probe A possesses excellent membrane permeability and low cytotoxicity, allowing for sensitive imaging of both exogenously supplemented Zn(II) ions in live cells, and endogenously releases Zn(II) ions in cells after treatment of 2,2-dithiodipyridine. MDPI 2019-04-22 /pmc/articles/PMC6515227/ /pubmed/31013675 http://dx.doi.org/10.3390/molecules24081592 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fang, Mingxi Xia, Shuai Bi, Jianheng Wigstrom, Travis P. Valenzano, Loredana Wang, Jianbo Tanasova, Marina Luck, Rudy L. Liu, Haiying Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes |
title | Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes |
title_full | Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes |
title_fullStr | Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes |
title_full_unstemmed | Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes |
title_short | Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes |
title_sort | detecting zn(ii) ions in live cells with near-infrared fluorescent probes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515227/ https://www.ncbi.nlm.nih.gov/pubmed/31013675 http://dx.doi.org/10.3390/molecules24081592 |
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