Hybrid gel electrophoresis using skin fibroblasts to aid in diagnosing mitochondrial disease

OBJECTIVE: We developed a novel, hybrid method combining both blue-native (BN-PAGE) and clear-native (CN-PAGE) polyacrylamide gel electrophoresis, termed BCN-PAGE, to perform in-gel activity stains on the mitochondrial electron transport chain (ETC) complexes in skin fibroblasts. METHODS: Four patie...

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Detalles Bibliográficos
Autores principales: Newell, Christopher, Khan, Aneal, Sinasac, David, Shoffner, John, Friederich, Marisa W., Van Hove, Johan L.K., Hume, Stacey, Shearer, Jane, Sosova, Iveta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515941/
https://www.ncbi.nlm.nih.gov/pubmed/31192304
http://dx.doi.org/10.1212/NXG.0000000000000336

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515941/
https://www.ncbi.nlm.nih.gov/pubmed/31192304
http://dx.doi.org/10.1212/NXG.0000000000000336

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