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NMNAT2‐mediated NAD(+) generation is essential for quality control of aged oocytes
Advanced maternal age has been reported to impair oocyte quality; however, the underlying mechanisms remain to be explored. In the present study, we identified the lowered NAD(+) content and decreased expression of NMNAT2 protein in oocytes from old mice. Specific depletion of NMNAT2 in mouse oocyte...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6516161/ https://www.ncbi.nlm.nih.gov/pubmed/30909324 http://dx.doi.org/10.1111/acel.12955 |
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author | Wu, Xinghan Hu, Feifei Zeng, Juan Han, Longsen Qiu, Danhong Wang, Haichao Ge, Juan Ying, Xiaoyan Wang, Qiang |
author_facet | Wu, Xinghan Hu, Feifei Zeng, Juan Han, Longsen Qiu, Danhong Wang, Haichao Ge, Juan Ying, Xiaoyan Wang, Qiang |
author_sort | Wu, Xinghan |
collection | PubMed |
description | Advanced maternal age has been reported to impair oocyte quality; however, the underlying mechanisms remain to be explored. In the present study, we identified the lowered NAD(+) content and decreased expression of NMNAT2 protein in oocytes from old mice. Specific depletion of NMNAT2 in mouse oocytes disturbs the meiotic apparatus assembly and metabolic activity. Of note, nicotinic acid supplementation during in vitro culture or forced expression of NMNAT2 in aged oocytes was capable of reducing the reactive oxygen species (ROS) production and incidence of spindle/chromosome defects. Moreover, we revealed that activation or overexpression of SIRT1 not only partly prevents the deficient phenotypes of aged oocytes but also ameliorates the meiotic anomalies and oxidative stress in NMNAT2‐depleted oocytes. To sum up, our data indicate a role for NMNAT2 in controlling redox homeostasis during oocyte maturation and uncover that NMNAT2‐ NAD(+)‐SIRT1 is an important pathway mediating the effects of maternal age on oocyte developmental competence. |
format | Online Article Text |
id | pubmed-6516161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65161612019-06-01 NMNAT2‐mediated NAD(+) generation is essential for quality control of aged oocytes Wu, Xinghan Hu, Feifei Zeng, Juan Han, Longsen Qiu, Danhong Wang, Haichao Ge, Juan Ying, Xiaoyan Wang, Qiang Aging Cell Original Papers Advanced maternal age has been reported to impair oocyte quality; however, the underlying mechanisms remain to be explored. In the present study, we identified the lowered NAD(+) content and decreased expression of NMNAT2 protein in oocytes from old mice. Specific depletion of NMNAT2 in mouse oocytes disturbs the meiotic apparatus assembly and metabolic activity. Of note, nicotinic acid supplementation during in vitro culture or forced expression of NMNAT2 in aged oocytes was capable of reducing the reactive oxygen species (ROS) production and incidence of spindle/chromosome defects. Moreover, we revealed that activation or overexpression of SIRT1 not only partly prevents the deficient phenotypes of aged oocytes but also ameliorates the meiotic anomalies and oxidative stress in NMNAT2‐depleted oocytes. To sum up, our data indicate a role for NMNAT2 in controlling redox homeostasis during oocyte maturation and uncover that NMNAT2‐ NAD(+)‐SIRT1 is an important pathway mediating the effects of maternal age on oocyte developmental competence. John Wiley and Sons Inc. 2019-03-25 2019-06 /pmc/articles/PMC6516161/ /pubmed/30909324 http://dx.doi.org/10.1111/acel.12955 Text en © 2019 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Papers Wu, Xinghan Hu, Feifei Zeng, Juan Han, Longsen Qiu, Danhong Wang, Haichao Ge, Juan Ying, Xiaoyan Wang, Qiang NMNAT2‐mediated NAD(+) generation is essential for quality control of aged oocytes |
title | NMNAT2‐mediated NAD(+) generation is essential for quality control of aged oocytes |
title_full | NMNAT2‐mediated NAD(+) generation is essential for quality control of aged oocytes |
title_fullStr | NMNAT2‐mediated NAD(+) generation is essential for quality control of aged oocytes |
title_full_unstemmed | NMNAT2‐mediated NAD(+) generation is essential for quality control of aged oocytes |
title_short | NMNAT2‐mediated NAD(+) generation is essential for quality control of aged oocytes |
title_sort | nmnat2‐mediated nad(+) generation is essential for quality control of aged oocytes |
topic | Original Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6516161/ https://www.ncbi.nlm.nih.gov/pubmed/30909324 http://dx.doi.org/10.1111/acel.12955 |
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