Can we use rapid lifetime determination for fast, fluorescence lifetime based, metabolic imaging? Precision and accuracy of double-exponential decay measurements with low total counts

Fluorescence lifetime imaging microscopy (FLIM) can assess cell’s metabolism through the fluorescence of the co-enzymes NADH and FAD, which exhibit a double-exponential decay, with components related to free and protein-bound conditions. In vivo real time clinical imaging applications demand fast ac...

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Detalles Bibliográficos
Autores principales: Silva, Susana Figueiredo, Domingues, José Paulo, Morgado, António Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6516636/
https://www.ncbi.nlm.nih.gov/pubmed/31086413
http://dx.doi.org/10.1371/journal.pone.0216894

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6516636/
https://www.ncbi.nlm.nih.gov/pubmed/31086413
http://dx.doi.org/10.1371/journal.pone.0216894

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