Optimization of tris(2-carboxyethyl) phosphine reduction conditions for fast analysis of total biothiols in mouse serum samples

In this study, we investigated suitable conditions for the reduction of disulfides in mouse serum samples by tris(2-carboxyethyl) phosphine (TCEP) for fast analysis of total biothiols. Disulfides were reduced with TCEP, and then, thiols were derivatized with the fluorogenic reagent, ammonium 7-fluor...

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Detalles Bibliográficos
Autores principales: Chang, Chunfang, Isokawa, Muneki, Funatsu, Takashi, Tsunoda, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517333/
https://www.ncbi.nlm.nih.gov/pubmed/31193090
http://dx.doi.org/10.1016/j.heliyon.2019.e01598

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517333/
https://www.ncbi.nlm.nih.gov/pubmed/31193090
http://dx.doi.org/10.1016/j.heliyon.2019.e01598

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