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Targeting mutations to the plastidial psbA gene of Chlamydomonas reinhardtii without direct positive selection

Targeting mutations to specific genomic loci is invaluable for assessing in vivo the effect of these changes on the biological role of the gene in study. Here, we attempted to introduce a mutation that was previously implicated in an increased heat stability of the mesophilic cyanobacterium Synechoc...

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Detalles Bibliográficos
Autores principales: Shmidt, Volha, Kaftan, David, Scherz, Avigdor, Danon, Avihai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517589/
https://www.ncbi.nlm.nih.gov/pubmed/31089169
http://dx.doi.org/10.1038/s41598-019-42617-9